Neurogenin2-d4Venus and Gadd45g-d4Venus transgenic mice: visualizing mitotic and migratory behaviors of cells committed to the neuronal lineage in the developing mammalian brain.
Neurogenin2-d4Venus and Gadd45g-d4Venus transgenic mice: visualizing mitotic and migratory behaviors of cells committed to the neuronal lineage in the developing mammalian brain.
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DOI：
10.1111/dgd.12131
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Miyata T
中科院分区：
文献类型：
--
作者：
Kawaue T;Sagou K;Kiyonari H;Ota K;Okamoto M;Shinoda T;Kawaguchi A;Miyata T
为了对哺乳动物大脑原基中神经元谱系细胞的形态和动力学进行高度敏感和全面的评估，我们生成了两个转基因小鼠品系，它们表达由 Neurogenin2 (Neurog2) 或 Gadd45g 基因的调控元件控制的不稳定 (d4) 金星。在胚胎中期新皮质壁中，Neurog2-d4Venus 的表达大部分与 Neurog2 蛋白的表达重叠，但发病时间略有延迟（1 小时）。尽管 Neurog2-d4Venus 和 Gadd45g-d4Venus 小鼠在心室区 (VZ) 表现出非常相似的标记模式，但在 Gadd45g-d4Venus 小鼠中，可以在包含完全分化神经元的更多基底区域中观察到细胞，而该区域不存在 Neurog2-d4Venus 荧光。延时监测显示，VZ 中的大多数 d4Venus+ 细胞都有延伸至顶端表面的突起；这些细胞中的许多最终缩回其顶端过程并从基底迁移到室下区，在那里神经元以及经历终末神经元产生分裂的中间神经源性祖细胞可以通过 d4Venus 荧光进行实时监测。一些 d4Venus+ VZ 细胞反而经历了核迁移到顶端表面，在那里它们分裂生成两个 d4Venus+ 子细胞，这表明在顶端表面产生神经元对的对称末端分裂可以得到可靠的实时监测。在其他发育中的神经区域（包括视网膜、脊髓和小脑）以及周围神经系统的区域（例如背根神经节）中也观察到了类似的谱系定向细胞。这些小鼠品系将有助于阐明哺乳动物神经系统发育的细胞和分子机制。
To achieve highly sensitive and comprehensive assessment of the morphology and dynamics of cells committed to the neuronal lineage in mammalian brain primordia, we generated two transgenic mouse lines expressing a destabilized (d4) Venus controlled by regulatory elements of the Neurogenin2 (Neurog2) or Gadd45g gene. In mid-embryonic neocortical walls, expression of Neurog2-d4Venus mostly overlapped with that of Neurog2 protein, with a slightly (1 h) delayed onset. Although Neurog2-d4Venus and Gadd45g-d4Venus mice exhibited very similar labeling patterns in the ventricular zone (VZ), in Gadd45g-d4Venus mice cells could be visualized in more basal areas containing fully differentiated neurons, where Neurog2-d4Venus fluorescence was absent. Time-lapse monitoring revealed that most d4Venus+ cells in the VZ had processes extending to the apical surface; many of these cells eventually retracted their apical process and migrated basally to the subventricular zone, where neurons, as well as the intermediate neurogenic progenitors that undergo terminal neuron-producing division, could be live-monitored by d4Venus fluorescence. Some d4Venus+ VZ cells instead underwent nuclear migration to the apical surface, where they divided to generate two d4Venus+ daughter cells, suggesting that the symmetric terminal division that gives rise to neuron pairs at the apical surface can be reliably live-monitored. Similar lineage-committed cells were observed in other developing neural regions including retina, spinal cord, and cerebellum, as well as in regions of the peripheral nervous system such as dorsal root ganglia. These mouse lines will be useful for elucidating the cellular and molecular mechanisms underlying development of the mammalian nervous system.
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影响因子：
16.2
作者：
Franco SJ;Müller U
通讯作者：
Müller U
影响因子：
2.7
作者：
Hufnagel RB;Le TT;Riesenberg AL;Brown NL
通讯作者：
Brown NL
DOI：
10.1073/pnas.0308600100
发表时间：
2004-03-02
影响因子：
11.1
作者：
Haubensak, W;Attardo, A;Huttner, WB
通讯作者：
Huttner, WB
影响因子：
64.5
作者：
Lui JH;Hansen DV;Kriegstein AR
通讯作者：
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影响因子：
5.3
作者：
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通讯作者：
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