A major root-associated acid phosphatase in Arabidopsis, AtPAP10, is regulated by both local and systemic signals under phosphate starvation.
A major root-associated acid phosphatase in Arabidopsis, AtPAP10, is regulated by both local and systemic signals under phosphate starvation.
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拟南芥中一种主要的根相关酸性磷酸酶 AtPAP10 在磷酸盐饥饿下受到局部和全身信号的调节
DOI：
10.1093/jxb/eru377
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发表时间：
2014-12
影响因子：
6.9
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Zhang Y;Wang X;Lu S;Liu D
在磷饥饿下，拟南芥酸性磷酸酶AtPAP10的根相关活性在转录、转录后和翻译后水平上受到局部和系统信号的差异调节。酸性磷酸酶(APase)的诱导和分泌是植物对磷(PI)饥饿的普遍反应。AtPAP10(拟南芥紫色酸性磷酸酶10)是一种主要的PI饥饿诱导的APase，与拟南芥的根表面相关。到目前为止，局部和系统信号在调节与根相关的AtPAP10活性中的作用在很大程度上仍不清楚。在这项工作中，我们证明了在蔗糖存在的情况下，局部和外部PI可用性的降低足以诱导AtPAP10在根中转录，蔗糖是一种来自芽的系统信号，而诱导的幅度取决于整个植物的PI状态。一旦AtPAP10mRNAs在根中合成，随后AtPAP10蛋白在根细胞中的积累和根表面AtPAP10活性的增加主要受局部信号的控制。此前，乙烯已被证明是AtPAP10活性的正向调节因子。在本研究中，我们提供的证据表明，在PI缺乏的情况下，乙烯主要调节根表面AtPAP10的酶活性，而不是AtPAP10的转录和蛋白质积累，这表明它作为局部信号发挥作用。此外，我们的工作表明，乙烯对根相关AtPAP10活性的诱导依赖于蔗糖，但蔗糖的作用不依赖于乙烯。这些结果揭示了局部和系统信号在PI饥饿下调控根相关AtPAP10活性中的不同作用。
Under phosphate starvation, the root-associated activity of Arabidopsis acid phosphatase AtPAP10 is differentially regulated by local and systemic signalling at transcriptional, post-transcriptional, and post-translational levels. The induction and secretion of acid phosphatases (APases) is a universal response of plants to phosphate (Pi) starvation. AtPAP10 (Arabidopsis purple acid phosphatase 10) is a major Pi starvation-induced APase that is associated with the root surface in Arabidopsis. So far, the roles of local and systemic signalling in regulating root-associated AtPAP10 activity remain largely unknown. In this work, we show that a decrease of local, external Pi availability is sufficient to induce AtPAP10 transcription in roots in the presence of sucrose, a systemic signal from shoots, whereas the magnitude of the induction is affected by the Pi status of the whole plant. Once the AtPAP10 mRNAs are synthesized in roots, subsequent accumulation of AtPAP10 proteins in root cells and increase in AtPAP10 activity on the root surface are mainly controlled by local signalling. Previously, ethylene has been demonstrated to be a positive regulator of AtPAP10 activity. In this study, we provide evidence that under Pi deficiency ethylene mainly modulates enzymatic activity of AtPAP10 on the root surface, but not AtPAP10 transcription and protein accumulation, suggesting that it functions as a local signal. Furthermore, our work indicates that the effect of ethylene on the induction of root-associated AtPAP10 activity depends on sucrose, but that the effect of sucrose does not depend on ethylene. These results reveal new insights into the distinct roles of local and systemic signalling in the regulation of root-associated AtPAP10 activity under Pi starvation.
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影响因子：
4.3
作者：
Karthikeyan, Athikkattuvalasu S.;Varadarajan, Deepa K.;Raghothama, Kashchandra G.
通讯作者：
Raghothama, Kashchandra G.
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