Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives.
Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives.
复制标题
DOI：
10.3390/genes12040556
复制
发表时间：
2021-04-12
期刊：
影响因子：
3.5
通讯作者：
Blair MW
中科院分区：
文献类型：
--
作者：
Buitrago-Bitar MA;Cortés AJ;López-Hernández F;Londoño-Caicedo JM;Muñoz-Florez JE;Muñoz LC;Blair MW
预计气候变化的一些主要影响将出现在干旱压力已经成为问题的地区。豆类作物一般对干旱敏感。然而，特帕里豆及其野生近亲尖叶菜豆（Phaseolus acutifolius）或小叶菜豆（P. parvifolius）来自墨西哥和美国之间的干旱地区。因此，我们假设这些豆类种质具有多样性信号，表明适应干旱的关键候选基因，如：Asr2，Dreb2B和ERECTA。通过对这些基因的等位基因进行测序，并与地理参考的特帕里豆种质收集地点的气候数据中的耐旱指数估计值进行比较，我们确定了每个基因的基因型x环境关联（GEA）。多样性分析发现，栽培尖叶海棠与野生尖叶海棠混杂在一起。tenuifolius和P.parvifolius的等位基因多样性，表明在广义（广义）评估中野生和栽培分支中的等位基因多样性是充足的。基因Dreb2B和ERECTA具有定向选择的特征，由与环境干旱指数相关的6个SNP表示。这表明，野生tepary豆是一个水库的新等位基因的耐旱性基因，作为一个物种，起源于干旱环境的预期。我们的研究证实，候选基因的方法是有效的标记验证在广泛的遗传基础的野生tepary加入。
Some of the major impacts of climate change are expected in regions where drought stress is already an issue. Grain legumes are generally drought susceptible. However, tepary bean and its wild relatives within Phaseolus acutifolius or P. parvifolius are from arid areas between Mexico and the United States. Therefore, we hypothesize that these bean accessions have diversity signals indicative of adaptation to drought at key candidate genes such as: Asr2, Dreb2B, and ERECTA. By sequencing alleles of these genes and comparing to estimates of drought tolerance indices from climate data for the collection site of geo-referenced, tepary bean accessions, we determined the genotype x environmental association (GEA) of each gene. Diversity analysis found that cultivated and wild P. acutifolius were intermingled with var. tenuifolius and P. parvifolius, signifying that allele diversity was ample in the wild and cultivated clade over a broad sense (sensu lato) evaluation. Genes Dreb2B and ERECTA harbored signatures of directional selection, represented by six SNPs correlated with the environmental drought indices. This suggests that wild tepary bean is a reservoir of novel alleles at genes for drought tolerance, as expected for a species that originated in arid environments. Our study corroborated that candidate gene approach was effective for marker validation across a broad genetic base of wild tepary accessions.
登录
查看更多内容
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
2.3
作者：
Carmenza Munoz, L.;Duque, Myriam C.;Blair, Matthew W.
通讯作者：
Blair, Matthew W.
影响因子：
5.6
作者：
Bitocchi E;Rau D;Bellucci E;Rodriguez M;Murgia ML;Gioia T;Santo D;Nanni L;Attene G;Papa R
通讯作者：
Papa R
影响因子：
3.7
作者：
Blair MW;Soler A;Cortés AJ
通讯作者：
Cortés AJ
DOI：
10.3390/plants9101299
发表时间：
2020-10-01
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Borelli T;Hunter D;Powell B;Ulian T;Mattana E;Termote C;Pawera L;Beltrame D;Penafiel D;Tan A;Taylor M;Engels J
通讯作者：
Engels J