Molecular ecology and selection in the drought-related Asr gene polymorphisms in wild and cultivated common bean (Phaseolus vulgaris L.).
Molecular ecology and selection in the drought-related Asr gene polymorphisms in wild and cultivated common bean (Phaseolus vulgaris L.).
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分子生态学和在野生和培养的共同豆类中与干旱相关的ASR基因多态性中的选择（叶状豆类叶）。
DOI：
10.1186/1471-2156-13-58
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发表时间：
2012-07-16
期刊：
影响因子：
2.9
通讯作者：
Blair MW
中科院分区：
文献类型：
--
作者：
Cortés AJ;Chavarro MC;Madriñán S;This D;Blair MW
脱落酸（ABA）途径在植物对干旱胁迫的反应中起着重要作用，ABA-胁迫响应（Asr）基因在这一过程中起着重要的调控作用。在此意义上，我们在栽培菜豆（Phaseolus vulgaris L.）参考资料和野生菜豆核心资料中获取了两个与ABA信号通路相关的耐旱性候选基因Asr1和Asr2的核苷酸多样性。我们的野生种群样本涵盖了一系列中度（半干旱）到非常干燥（沙漠）的栖息地，而我们的栽培样本则表现出广泛的耐旱性。这两个基因的核苷酸变异模式非常不同。与先前在这些人群中调查的中性参考位点相比，Asr1显示出非常低的核苷酸多样性。这表明强烈的净化选择作用于该基因。相比之下，Asr2表现出更高水平的核苷酸多样性，这表明了适应选择。这些模式在野生豆中比在栽培的普通豆中更为显著，这表明与驯化以来的农民选择相比，自然选择在很长一段时间内发挥了作用。总之，这些结果表明Asr1在耐旱性方面的重要性，并为该基因家族遗传多态性与耐旱性性状变异之间的关联研究迈出了第一步。此外，我们的主要成功之一是发现野生普通豆是Asr基因的遗传变异和选择特征的储存库，这可能对栽培普通豆的耐旱性育种有用。
The abscisic acid (ABA) pathway plays an important role in the plants’ reaction to drought stress and ABA-stress response (Asr) genes are important in controlling this process. In this sense, we accessed nucleotide diversity at two candidate genes for drought tolerance (Asr1 and Asr2), involved in an ABA signaling pathway, in the reference collection of cultivated common bean (Phaseolus vulgaris L.) and a core collection of wild common bean accessions. Our wild population samples covered a range of mesic (semi-arid) to very dry (desert) habitats, while our cultivated samples presented a wide spectrum of drought tolerance. Both genes showed very different patterns of nucleotide variation. Asr1 exhibited very low nucleotide diversity relative to the neutral reference loci that were previously surveyed in these populations. This suggests that strong purifying selection has been acting on this gene. In contrast, Asr2 exhibited higher levels of nucleotide diversity, which is indicative of adaptive selection. These patterns were more notable in wild beans than in cultivated common beans indicting that natural selection has played a role over long time periods compared to farmer selection since domestication. Together these results suggested the importance of Asr1 in the context of drought tolerance, and constitute the first steps towards an association study between genetic polymorphism of this gene family and variation in drought tolerance traits. Furthermore, one of our major successes was to find that wild common bean is a reservoir of genetic variation and selection signatures at Asr genes, which may be useful for breeding drought tolerance in cultivated common bean.
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影响因子：
9.8
作者：
Boyko AR;Quignon P;Li L;Schoenebeck JJ;Degenhardt JD;Lohmueller KE;Zhao K;Brisbin A;Parker HG;vonHoldt BM;Cargill M;Auton A;Reynolds A;Elkahloun AG;Castelhano M;Mosher DS;Sutter NB;Johnson GS;Novembre J;Hubisz MJ;Siepel A;Wayne RK;Bustamante CD;Ostrander EA
通讯作者：
Ostrander EA
影响因子：
2.3
作者：
Beebe, S;Rengifo, J;Tohme, J
通讯作者：
Tohme, J
影响因子：
3.1
作者：
ANDERSON, JA;CHURCHILL, GA;SORRELLS, ME
通讯作者：
SORRELLS, ME
影响因子：
9.4
作者：
Fischer, Iris;Camus-Kulandaivelu, Letizia;Stephan, Wolfgang
通讯作者：
Stephan, Wolfgang
DOI：
10.1007/s11032-010-9527-9
发表时间：
2012-01
期刊：
Molecular breeding : new strategies in plant improvement
影响因子：
--
作者：
Blair MW;Galeano CH;Tovar E;Muñoz Torres MC;Castrillón AV;Beebe SE;Rao IM
通讯作者：
Rao IM