Immediate Effects of Maternal Deprivation on the (Re)Activity of the HPA-Axis Differ in CD1 and C57Bl/6J Mouse Pups.
Immediate Effects of Maternal Deprivation on the (Re)Activity of the HPA-Axis Differ in CD1 and C57Bl/6J Mouse Pups.
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DOI：
10.3389/fendo.2014.00190
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发表时间：
2014
影响因子：
5.2
通讯作者：
Oitzl MS
中科院分区：
文献类型：
--
作者：
Daskalakis NP;Enthoven L;Schoonheere E;de Kloet ER;Oitzl MS
小鼠出生后发育的特征是下丘脑-垂体-肾上腺（HPA）轴对轻度应激的低反应性。在此期间，母亲剥夺（MD）可以破坏HPA轴的静止。本研究探讨了影响的菌株（远交系CD 1与近交系C57 BL/6 J小鼠）的HPA轴的新生小鼠（5天）在其母亲的存在下，或24小时MD（出生后第4天）在基础或轻度应激条件下的一些中央和外周组件。在母鼠存在的情况下，两种小鼠品系的肾上腺皮质酮（CORT）分泌均较低。与CD 1小鼠相比，C57 BL/6 J具有与较高ACTH水平和ACTH/CORT比率相关的较低CORT水平（即，室旁核糖皮质激素受体（GR）mRNA表达增高。虽然MD disinhibit HPA轴在这两个菌株所反映的增加基础皮质醇和促肾上腺皮质激素，我们发现一个应变依赖的模式。与CD 1小鼠相比，MD使C57 BL/6 J中的CORT增加更多，同时ACTH/CORT比率较低（即，高肾上腺对ACTH的敏感性），而GR mRNA在两种菌株中不再有差异。然而，这种增加的肾上腺敏感性在母亲剥夺C57 BL/6 J小鼠没有反映在他们的CORT反应，随后的新奇的压力，可能是由于MD诱导的天花板效应，在他们的类固醇生成能力。总之，MD的直接结果取决于母婴二分体的遗传背景，这表明可能后期生活中的结果也不能一概而论。
The postnatal development of the mouse is characterized by a period of hypo-responsiveness of the hypothalamic–pituitary–adrenal (HPA) axis to mild stressors. Maternal deprivation (MD) during this period can disrupt the quiescence of the HPA-axis. The present study examined the influence of strain (outbred CD1 vs. inbred C57BL/6J mice) on some central and peripheral components of the HPA-axis in neonatal mice (5-day-old) in the presence of their mother or after 24 h MD (on postnatal day 4) under basal or mild stressful conditions. In the presence of the dam, adrenal corticosterone (CORT) secretion was low in both mouse strains. Compared to CD1 mice, C57BL/6J had lower CORT levels associated with higher ACTH levels and ACTH/CORT ratio (i.e., lower adrenal sensitivity to ACTH), and higher glucocorticoid receptor (GR) mRNA expression in the paraventricular nucleus. Although MD disinhibited the HPA-axis in both strains as reflected by increased basal CORT and ACTH, we found a strain-dependent pattern. MD increased CORT more in C57BL/6J compared to CD1 mice together with a lower ACTH/CORT ratio (i.e., higher adrenal sensitivity to ACTH), while GR mRNA was no longer different in the two strains. However, this increased adrenal sensitivity in maternally deprived C57BL/6J mice was not reflected in their CORT response to a subsequent novelty stressor, possibly due to an MD-induced ceiling effect in their steroidogenic capacity. In conclusion, the immediate outcome of MD depends on the genetic background of the mother–infant dyad, suggesting that maybe also the outcome in later-life cannot be generalized.
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DOI：
10.1017/s1461145703003729
发表时间：
2003-12-01
影响因子：
4.8
作者：
MacQueen, GM;Ramakrishnan, K;Young, LT
通讯作者：
Young, LT
影响因子：
2.9
作者：
Hotchkiss, AK;Pyter, LM;Nelson, RJ
通讯作者：
Nelson, RJ
影响因子：
2.9
作者：
Roedel, Heiko G.;Meyer, Susann;Hudson, Robyn
通讯作者：
Hudson, Robyn
影响因子：
8.2
作者：
Kosten, Therese A.;Kim, Jeansok J.;Lee, Hongjoo J.
通讯作者：
Lee, Hongjoo J.
影响因子：
3.7
作者：
Daskalakis, Nikolaos P.;Bagot, Rosemary C.;Parker, Karen J.;Vinkers, Christiaan H.;de Kloet, E. R.
通讯作者：
de Kloet, E. R.