Persistence of clinically relevant levels of SARS-CoV2 envelope gene subgenomic RNAs in non-immunocompromised individuals.
Persistence of clinically relevant levels of SARS-CoV2 envelope gene subgenomic RNAs in non-immunocompromised individuals.
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DOI：
10.1016/j.ijid.2021.12.312
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Harries LW
中科院分区：
文献类型：
--
作者：
Davies M;Bramwell LR;Jeffery N;Bunce B;Lee BP;Knight B;Auckland C;Masoli JA;Harries LW
本研究旨在评估 COVID-19 严重程度与活跃病毒载量之间的关联，并表征从英国西南部第一波 COVID-19 感染患者身上采集的一系列档案样本中活跃 SARS-CoV-2 清除的动态。在来自 176 名个体的 PCR 确认的 SARS-CoV-2 阳性样本的回顾性收集中测量了亚基因组 RNA (sgRNA) 和 E 基因基因组序列，并与疾病严重程度相关。然后根据症状出现和最后一次阳性检测来评估病毒清除动态。虽然 E 基因 sgRNA 在 E 基因基因组序列之前下降，但一些个体保留 sgRNA 阳性长达 68 天。 13% 的 sgRNA 阳性病例在 10 天后仍表现出临床相关的病毒水平，之前没有与病毒清除时间延长相关的临床特征。我们的结果表明，潜在活跃的病毒有时可能持续超过 10 天，并可能构成进一步传播的潜在风险。如果这将构成严重的公共卫生威胁，则可能需要采取额外的缓解策略，以降低脆弱环境中继发病例的风险。
This study aimed to evaluate the associations between COVID-19 severity and active viral load, and to characterize the dynamics of active SARS-CoV-2 clearance in a series of archival samples taken from patients in the first wave of COVID-19 infection in the South West of the UK. Subgenomic RNA (sgRNA) and E-gene genomic sequences were measured in a retrospective collection of PCR-confirmed SARS-CoV-2-positive samples from 176 individuals, and related to disease severity. Viral clearance dynamics were then assessed in relation to symptom onset and last positive test. Whilst E-gene sgRNAs declined before E-gene genomic sequences, some individuals retained sgRNA positivity for up to 68 days. 13% of sgRNA-positive cases still exhibited clinically relevant levels of virus after 10 days, with no clinical features previously associated with prolonged viral clearance times. Our results suggest that potentially active virus can sometimes persist beyond a 10-day period, and could pose a potential risk of onward transmission. Where this would pose a serious public health threat, additional mitigation strategies may be necessary to reduce the risk of secondary cases in vulnerable settings.
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影响因子：
11.1
作者：
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通讯作者：
Ke, Changwen
影响因子：
5.4
作者：
Dagotto, Gabriel;Mercado, Noe B.;Barouch, Dan H.
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作者：
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通讯作者：
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影响因子：
16.6
作者：
Alexandersen S;Chamings A;Bhatta TR
通讯作者：
Bhatta TR
DOI：
10.4269/ajtmh.20-1110
发表时间：
2020-12
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Kwon JS;Kim JY;Kim MC;Park SY;Kim BN;Bae S;Cha HH;Jung J;Kim MJ;Lee MJ;Choi SH;Chung JW;Shin EC;Kim SH
通讯作者：
Kim SH