Mild traumatic brain injury (MTBI) leads to spatial learning deficits.
Mild traumatic brain injury (MTBI) leads to spatial learning deficits.
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DOI：
10.3109/02699052.2011.635362
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发表时间：
2012
期刊：
影响因子：
1.9
通讯作者：
Therrien B
中科院分区：
文献类型：
--
作者：
Darwish H;Mahmood A;Schallert T;Chopp M;Therrien B
本研究旨在探讨轻度和重度TBI对幼年雄性Wistar大鼠空间学习能力的影响。采用随机重复测量实验设计，在三个独立的动物组中检查空间学习。研究中包括24只（重度n = 9，轻度n = 8，假手术n = 7）雄性大鼠。动物接受对照轻度（1.5 mm）、重度（2.5 mm）皮质撞击损伤或假手术。创伤后20天，连续5天，每天使用改良的Morris水迷宫测试评估空间学习。计算在每个象限内行进的时间百分比和逃避潜伏期。使用神经元（MAP 2）和突触前蛋白（突触素）生物标志物在损伤后检查所有动物的海马脑区。轻度损伤的动物直到第3天才到达平台;轻度和重度损伤的动物在目标象限的时间明显少于假手术组。重度损伤组和轻度损伤组海马神经元数目差异有统计学意义（P < 0. 05），但重度损伤组和轻度损伤组齿状回突触体蛋白密度百分比均显著低于假手术组。轻度TBI后存在持续的空间学习缺陷，这些缺陷似乎相当于更严重的损伤后表现出的缺陷。
The aim of this study was to investigate the effect of mild and severe TBI on young male Wistar rats' spatial learning. Randomized repeated measure experimental design was used to examine spatial learning in three independent animal groups. Twenty-four (severe n = 9, mild n = 8, sham n = 7) male rats were included in the study. Animals received controlled mild (1.5 mm), severe (2.5 mm) cortical impact injury or sham surgery. Spatial learning was assessed daily using a modified Morris water maze test, 20 days post-trauma, for 5 consecutive days. Percentage time travelled within each quadrant and escape latency were calculated. All animals' hippocampal brain regions were examined post-injury using neuron (MAP2) and pre-synaptic protein (Synaptophysin) biomarkers. It took the animals with mild injury until day 3 to reach the platform; and animals with mild and severe injury spent significantly less time in the target quadrant than the sham. The hippocampal neuron numbers differed proportionately between animals with severe and mild injury, but the percentage of synaptophysin density was significantly less in the dentate gyrus of both animals with mild and severe injury than sham group. Persistent spatial learning deficits exist after mild TBI; these deficits appear equivalent to deficits exhibited after a more severe injury.
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