Changes in Distribution of Active Mitochondria during Oocyte Maturation and Fertilization in the Hamster
Changes in Distribution of Active Mitochondria during Oocyte Maturation and Fertilization in the Hamster
复制标题
仓鼠卵母细胞成熟和受精过程中活性线粒体分布的变化
DOI：
10.1274/jmor.22.163
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
K. Toyokawa
中科院分区：
文献类型：
--
作者：
Hiroyuki Suzuki;M. Satoh;K. Toyokawa
用罗丹明123（Rh 123）、线粒体示踪红（MT-Red）和碘化3，3 ′-二己基氧碳菁（DiOC 6（3））三种荧光探针对仓鼠卵母细胞成熟和受精过程中线粒体的定位和活性进行了监测。还检查了体内老化的卵母细胞。生发泡（GV）卵母细胞表现出一系列的线粒体组织，从均匀分布到更皮质的限制。中期I（MI）和MII卵母细胞包括大量的线粒体，其中的荧光强度增加了两倍，GV卵母细胞。大约一半（54.5%）的MII卵母细胞表现出线粒体自由区的皮质。在排卵后10小时的卵母细胞中观察到线粒体密度下降约10%，尽管没有显著变化。受精后，线粒体逐渐聚集并定位于所有检查的卵子的核周区域。这些比较研究表明，Rh 123和MT-Red的信号几乎一致，但DiOC 6（3）的信号显示出更宽的明亮组织区域，而不管本研究中使用的浓度是否非常低（5 ng/ml）。此外，从一个相对均匀的分布活跃的线粒体核周群集的过渡可能反映了能量的生产和利用过程中的卵母细胞成熟和受精在仓鼠，在其他物种的报告。
ABSTRACT The localization and activity of mitochondria in hamster oocytes during maturation and fertilization were monitored by using three different fluorescent probes: rhodamine 123 (Rh123), MitoTracker Red (MT-Red) and 3,3′-dihexyloxacarbocyanine iodide (DiOC6(3)). Oocytes aged in vivo were also examined. The germinal vesicle (GV) oocytes exhibited a range of mitochondrial organizations, from a uniform distribution to a more cortical restriction. Metaphase I (MI) and MII oocytes included numerous mitochondria, among which the fluorescence intensity increased two-fold over that of GV oocytes. About half (54.5%) of the MII oocytes exhibited a mitochondria free zone at the cortex. An approximately 10 % decline in the density of mitochondria was observed in the oocytes aged 10 h post-ovulation, despite no significant changes. After fertilization, mitochondria became progressively aggregated and localized in the perinuclear region of all eggs examined. These comparative studies show that the signals for Rh123 and MT-Red were virtually coincident, but that for DiOC6(3) displayed wider areas of bright organizations regardless of the very low concentration used in this study (5 ng/ml). In addition, transition from a relatively homogeneous distribution of active mitochondria to perinuclear clustering may reflect energy production and utilization during oocyte maturation and fertilization in hamsters, as reported in other species.
影响因子：
6.1
作者：
McKiernan, SH;Bavister, BD
通讯作者：
Bavister, BD
影响因子：
2.7
作者：
ALLWORTH, AE;ALBERTINI, DF
通讯作者：
ALBERTINI, DF