Development of high-productivity, strong cation-exchange adsorbers for protein capture by graft polymerization from membranes with different pore sizes.
Development of high-productivity, strong cation-exchange adsorbers for protein capture by graft polymerization from membranes with different pore sizes.
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DOI：
10.1016/j.memsci.2012.07.040
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发表时间：
2012-12-15
影响因子：
9.5
通讯作者：
Husson, Scott M.
中科院分区：
文献类型：
--
作者：
Chenette, Heather C. S.;Robinson, Julie R.;Hobley, Eboni;Husson, Scott M.
本文介绍了使用ATRP（原子转移自由基聚合）的大孔膜的表面改性，以创建具有高蛋白质结合能力和高产品通量的阳离子交换吸附剂。这项工作的动机是在蛋白质治疗剂的下游加工中需要更经济和快速的捕获步骤。用聚（甲基丙烯酸3-磺丙酯，钾盐）触角修饰具有三种报告的标称孔径（0.2，0.45，1.0 μm）的膜，以产生高密度的蛋白质结合位点。使用了一种特殊的配方，其中单体被冠醚保护，以使这种阳离子聚合物能够进行表面引发的ATRP。通过使用傅里叶变换红外光谱的化学分析和通过测量作为聚合时间的函数的纯水通量间接地支持改性的成功。用共聚焦激光扫描显微镜观察膜内修饰的均匀性。静态和动态的结合能力进行了测量，使用溶菌酶蛋白质，以允许与商业阳离子交换材料的性能数据进行比较。动态结合能力进行了测量，流速范围从13至109柱体积（CV）/分钟。结果表明，这种独特的ATRP配方可用于制造阳离子交换膜吸附器的动态结合能力高达70毫克/毫升，在100 CV/分钟的吞吐量和前所未有的生产率为300毫克/毫升/分钟。
This paper describes the surface modification of macroporous membranes using ATRP (atom transfer radical polymerization) to create cation-exchange adsorbers with high protein binding capacity at high product throughput. The work is motivated by the need for a more economical and rapid capture step in downstream processing of protein therapeutics. Membranes with three reported nominal pore sizes (0.2, 0.45, 1.0 μm) were modified with poly(3-sulfopropyl methacrylate, potassium salt) tentacles, to create a high density of protein binding sites. A special formulation was used in which the monomer was protected by a crown ether to enable surface-initiated ATRP of this cationic polyelectrolyte. Success with modification was supported by chemical analysis using Fourier-transform infrared spectroscopy and indirectly by measurement of pure water flux as a function of polymerization time. Uniformity of modification within the membranes was visualized with confocal laser scanning microscopy. Static and dynamic binding capacities were measured using lysozyme protein to allow comparisons with reported performance data for commercial cation-exchange materials. Dynamic binding capacities were measured for flow rates ranging from 13 to 109 column volumes (CV)/min. Results show that this unique ATRP formulation can be used to fabricate cation-exchange membrane adsorbers with dynamic binding capacities as high as 70 mg/mL at a throughput of 100 CV/min and unprecedented productivity of 300 mg/mL/min.
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影响因子：
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