Protective role of TNF-α, IL-10 and IL-2 in mice infected with the Oshima strain of Tick-borne encephalitis virus.
Protective role of TNF-α, IL-10 and IL-2 in mice infected with the Oshima strain of Tick-borne encephalitis virus.
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DOI：
10.1038/srep05344
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发表时间：
2014-06-18
影响因子：
4.6
通讯作者：
Hayasaka D
中科院分区：
文献类型：
--
作者：
Tun MM;Aoki K;Senba M;Buerano CC;Shirai K;Suzuki R;Morita K;Hayasaka D
森林脑炎病毒(TBEV)可引起急性中枢神经系统疾病。在这里，我们研究了肿瘤坏死因子-α、白介素10和其他细胞因子在感染大岛株和软津株后在KO小鼠中的作用。感染大岛毒株后，与野生型(WT)小鼠相比，α-KO和IL-10KO小鼠的死亡率显著增加。提示肿瘤坏死因子-α和IL-10对Oshima株致死性感染具有保护作用。然而，α-KO和IL-10KO小鼠与WT小鼠相比，脑内病毒载量和促炎细胞因子水平无显著差异。另一方面，WT、肿瘤坏死因子-αKO和IL-10 KO小鼠在感染Sofjin株后全部死亡。有趣的是，Sofjin感染的小鼠并没有表现出所有组小鼠脾中IL-2的mRNA水平上调，而大岛感染的小鼠与模拟感染的小鼠相比，IL-2的水平显著增加。这些结果提示，肿瘤坏死因子-α、IL-10和IL-2可能是脑炎患者脑炎缓解的关键因素。
Tick-borne encephalitis virus (TBEV) causes acute central nervous system disease. Here, we investigated the roles of the TNF-α, IL-10 and other cytokines in appropriate KO mice following infection with Oshima and Sofjin strains of TBEV. Following infection with the Oshima strain, mortality rates were significantly increased in TNF-α KO and IL-10 KO mice compared with wild type (WT) mice. These results suggested that TNF-α and IL-10 play protective roles against fatal infection due to Oshima strain infection. However, viral loads and proinflammatory cytokine levels in the brain of TNF-α KO andIL-10 KO mice were not significantly different compared with those of WT mice. On the other hand, all WT, TNF-α KO and IL-10 KO mice died following infection with Sofjin strain. Interestingly, Sofjin-infected mice did not exhibit an up-regulated mRNA level of IL-2 in the spleen in all groups of mice, whereas Oshima-infected mice showed significantly increased level of IL-2 compared with mock-infected mice. From these results, we suggest that TNF-α, IL-10 and IL-2 are key factors for disease remission from fatal encephalitis due to infection with Oshima strain of TBEV.
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