Expression study of soybean germin-like gene family reveals a role of GLP7 gene in various abiotic stress tolerances
Expression study of soybean germin-like gene family reveals a role of GLP7 gene in various abiotic stress tolerances
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大豆胚芽样基因家族的表达研究揭示了GLP7基因在各种非生物胁迫耐受性中的作用
DOI：
10.1139/cjps-2015-0213
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发表时间：
2016-04
影响因子：
1.2
通讯作者：
Wenbin Li
中科院分区：
文献类型：
--
作者：
Tao Wang;Mo Lu;Zhiwei Qin;Wenbin Li
摘要Germin-like蛋白(GLP)是一种广泛存在的植物糖蛋白(属于Cupin超家族)，在许多植物中发挥着不同的作用，包括非生物胁迫抗性。为了确定非生物胁迫响应的分子功能，分析了大豆GmGLPs类Germin蛋白编码基因的表达。对大豆叶片组织中21个GmGLPs转录本的丰度进行了QRT-PCR分析。结果表明，在高盐度、PEG6000、脱落酸(ABA)和甲基紫精(MV)处理下，GmGLPs转录本含量较高。聚乙二醇6000和氯化钠诱导的基因转录丰度高峰大多出现在胁迫后18h，部分基因表达早于胁迫后4h。进一步鉴定了一个在盐分、干旱、ABA和MV条件下高度丰富的GmGLP7基因。与野生型相比，GmGLP7(Glyma.08G226800.1)在转基因拟南芥中的异位过表达提高了对干旱、盐分和氧化的耐性，并导致了对ABA介导的种子萌发和初级根伸长的敏感表型。综上所述，这些结果表明GmGLP7在植物中具有积极的非生物耐受性。
Abstract Germin-like proteins (GLPs) are ubiquitous plant glycoproteins (belonging to the cupin super family) that play diverse roles, including abiotic stress resistance in many plant species. To identify the molecular functions underlying abiotic stress responses, the expression of germin-like protein encoding genes of soybean GmGLPs was analyzed. qRT-PCR analyses of 21 GmGLPs transcripts abundances were conducted in soybean leave tissues. The results showed that GmGLPs transcripts were highly abundant upon treatments with high salinity, PEG6000, abscisic acid (ABA) and methyl viologen (MV). The peaks of transcript copiousness induced by PEG6000 and NaCl were mostly observed after 18 h, while some genes expressed earlier than 4 h after abiotic stress treatment. A specific GmGLP7 gene, that was highly abundant under salinity, drought, ABA and MV conditions, was further characterized. The ectopic overexpression of GmGLP7 (Glyma.08G226800.1) in transgenic Arabidopsis enhanced drought, salt, and oxidative tolerance and resulted in hypersensitive phenotypes toward ABA-mediated seed germination and primary root elongation, compared to the wild-type. Taken together, these results suggest that GmGLP7 positively confers abiotic tolerance in plants.
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影响因子：
5.1
作者：
Carter, C;Graham, RA;Thornburg, RW
通讯作者：
Thornburg, RW
DOI：
10.1104/pp.107.3.737
发表时间：
1995-03
期刊：
--
影响因子：
--
作者：
L. Slooten;K. Capiau;W. V. Camp;M. V. Montagu;Chris Sybesma;Dirk;lnzé
通讯作者：
L. Slooten;K. Capiau;W. V. Camp;M. V. Montagu;Chris Sybesma;Dirk;lnzé
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1016/s0021-9258(18)99247-1
发表时间：
1991-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Lane;F. Bernier;E. Dratewka-Kos;R. Shafai;T. Kennedy;C. Pyne;J. Munro;T. Vaughan;D. Walters;F. Altomare
通讯作者：
B. Lane;F. Bernier;E. Dratewka-Kos;R. Shafai;T. Kennedy;C. Pyne;J. Munro;T. Vaughan;D. Walters;F. Altomare
影响因子：
4.3
作者：
Hamel, F;Breton, C;Houde, M
通讯作者：
Houde, M