Serum circulating cell free DNA as potential diagnostic and prognostic biomarker in non small cell lung cancer.
Serum circulating cell free DNA as potential diagnostic and prognostic biomarker in non small cell lung cancer.
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DOI：
10.1016/j.bbrep.2018.06.002
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发表时间：
2018-09
影响因子：
2.7
通讯作者：
Ibrahem RAL
中科院分区：
文献类型：
--
作者：
Soliman SE;Alhanafy AM;Habib MSE;Hagag M;Ibrahem RAL
非小细胞肺癌(NSCLC)是导致癌症相关死亡的主要原因，而NSCLC患者的存活率仍然很低，因此早期诊断NSCLC是治愈的最佳时机。无细胞DNA(cf-DNA)是存在于人的无细胞血浆/血清中的细胞外核酸，鉴于最近肺癌液体活检的批准，循环肿瘤DNA作为一种新的非侵入性诊断和预后生物标志物的应用前景广阔。探讨血清循环细胞游离DNA浓度能否作为非小细胞肺癌患者诊断和预后的生物标志物。本研究对60例非小细胞肺癌患者、40例慢性阻塞性肺疾病(COPD)患者和40例健康人进行了研究。血清循环cf-DNA定量分析采用Alu实时荧光定量聚合酶链式反应。采用双抗体夹心法测定血清CEA水平。NSCLC患者的ALU 215、ALU 247和DNA完整性均显著高于COPD患者和对照组。在ROC曲线分析中，ALU 247、ALU 115、DNA完整性(325、565、0.48)的总准确率分别为92.1%、83.6%、56.4%。结合DNA完整性和CEA，敏感性提高到93.3%。在非小细胞肺癌患者中，ALU 115和ALU 247随临床分期的进展而显著升高，且以转移性患者最高。在存活率方面，DNA完整性低的患者总体存活率较好。血清cf-DNA浓度和完整性指数可作为NSCLC早期诊断和预后判断的有价值的工具。
Non-small cell lung cancer (NSCLC) is leading cause of cancer related death and the survival rate for patients with NSCLC remain poor so early diagnosis of NSCLC represents the best opportunity for cure. Cell-free DNA (cf-DNA) is extracellular nucleic acids found in cell-free plasma/serum of humans, given the recent approval of a liquid biopsy in lung cancer, the use of circulating tumor DNA as a novel non-invasive diagnostic and prognostic biomarker is promising. Studying whether the concentrations of circulating Cell Free DNA in serum can be used as a diagnostic and prognostic biomarker for NSCLC patients. This study was carried out on 140 subjects included 60 patients with non small cell lung cancer,40 patients with Chronic Obstructive Pulmonary Disease (COPD) and 40 healthy controls. Quantitative analysis of serum circulating cf-DNA was done b y AlU-based quantitative real time PCR. Serum level of CEA was measured by ELISA. NSCLC patients demonstrated significantly higher values of each of ALU 215, ALU 247, and DNA integrity than both COPD patients and controls. On ROC curve analysis, the total accuracy of ALU 247, ALU 115, DNA integrity (92.1%, 83.6%, 56.4%) at cutoff points (325, 565 & 0.48) respectively. On combining both DNA integrity and CEA, improved sensitivity to 93.3% was noted. For NSCLC patients, ALU 115 & ALU 247 increased significantly with more advanced stage and highest level was noticed in metastatic patients. Regarding survival there was better overall survival among patients with low DNA integrity. Serum cf-DNA concentrations and integrity index may be valuable tool in early diagnosis of NSCLC and prediction of prognosis of those patients.
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影响因子：
4.1
作者：
Yoon, Kyong-Ah;Park, Sohee;Lee, Jin Soo
通讯作者：
Lee, Jin Soo
影响因子：
1
作者：
Zaher, Ebtsam R.;Anwar, Medhat M.;Abo-El-Eneen, Marwa S.
通讯作者：
Abo-El-Eneen, Marwa S.
影响因子：
3.4
作者：
da Silva Filho, Benisio Ferreira;Almeida Diniz Gurgel, Ana Pavla;Ferreira Silva, Luiz Antonio
通讯作者：
Ferreira Silva, Luiz Antonio
DOI：
10.6004/jnccn.2012.0022
发表时间：
2012-02
期刊：
Journal of the National Comprehensive Cancer Network : JNCCN
影响因子：
--
作者：
Wood DE;Eapen GA;Ettinger DS;Hou L;Jackman D;Kazerooni E;Klippenstein D;Lackner RP;Leard L;Leung AN;Massion PP;Meyers BF;Munden RF;Otterson GA;Peairs K;Pipavath S;Pratt-Pozo C;Reddy C;Reid ME;Rotter AJ;Schabath MB;Sequist LV;Tong BC;Travis WD;Unger M;Yang SC
通讯作者：
Yang SC
血清中循环游离 DNA 作为结直肠癌诊断和预后预测的生物标志物
影响因子：
8.8
作者：
Hao, T. B.;Shi, W.;Shen, X. J.;Qi, J.;Wu, X. H.;Wu, Y.;Tang, Y. Y.;Ju, S. Q.
通讯作者：
Ju, S. Q.