Circulating cell-free DNA in serum as a biomarker for diagnosis and prognostic prediction of colorectal cancer.
Circulating cell-free DNA in serum as a biomarker for diagnosis and prognostic prediction of colorectal cancer.
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血清中循环游离 DNA 作为结直肠癌诊断和预后预测的生物标志物
DOI：
10.1038/bjc.2014.470
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发表时间：
2014-10-14
影响因子：
8.8
通讯作者：
Ju, S. Q.
中科院分区：
文献类型：
--
作者：
Hao, T. B.;Shi, W.;Shen, X. J.;Qi, J.;Wu, X. H.;Wu, Y.;Tang, Y. Y.;Ju, S. Q.
背景：验证血清循环无细胞DNA （ccf-DNA）浓度和完整性指数是否可用于临床对结直肠癌（CRC）患者的诊断和进展监测。方法：收集原发性结直肠癌患者104例，手术性结直肠癌患者85例，复发/转移性结直肠癌患者16例，肠息肉患者63例，正常对照110例。采用实时荧光定量PCR扩增ALU重复序列（ALU- qpcr），检测血清中长（247 bp）和短（115 bp） DNA片段。采用ARCHITECT法检测血清癌胚抗原（CEA）水平。结果：原发性结直肠癌组中位绝对血清ALU115和ALU247/115显著高于肠息肉组和正常对照组（P均< 0.0001），复发/转移性结直肠癌组中ALU115和ALU247/115显著高于原发性结直肠癌组（P= 0.0021， P= 0.0018）或手术后结直肠癌组（P< 0.0001），随访中ALU115和ALU247/115术前升高，术后显著降低。结论：ALU115、ALU247/115与CEA联合检测可提高结直肠癌的诊断效率。血清DNA浓度和完整性指数对早期补充诊断、监测结直肠癌的进展和预后可能有价值。
Background:To verify whether the concentrations and integrity index of circulating cell-free DNA (ccf-DNA) in serum may be clinically useful for the diagnosis and progression monitoring of colorectal cancer (CRC) patients.Methods:Serum samples were collected from 104 with primary CRC, 85 with operated CRC, 16 with recurrent/metastatic CRC, 63 patients with intestinal polyps and 110 normal controls. Long (247 bp) and short (115 bp) DNA fragments in serum were detected by real-time quantitative PCR by amplifying the ALU repeats (ALU-qPCR). Serum carcinoembryonic antigen (CEA) level was detected by ARCHITECT assay.Results:The median absolute serum ALU115 and ALU247/115 in primary CRC group was significantly higher than those in intestinal polyp and normal control groups (both P< 0.0001), in recurrent/metastatic CRC was significantly higher compared with primary CRC (P= 0.0021, P= 0.0018) or operated CRC (P< 0.0001, respectively) and during follow-up, ALU115 and ALU247/115 were increased before surgery and decreased significantly after surgery.Conclusions:Combined detection of ALU115, ALU247/115 and CEA could improve the diagnostic efficiency for CRC. Serum DNA concentrations and integrity index may be valuable in early complementary diagnosis and monitoring of progression and prognosis of CRC.
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影响因子：
8.8
作者：
Mead, R.;Duku, M.;Bhandari, P.;Cree, I. A.
通讯作者：
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