Hepatitis B virus genotypes, expression quantitative trait loci for ZNRD1-AS1 and their interactions in hepatocellular carcinoma.
Hepatitis B virus genotypes, expression quantitative trait loci for ZNRD1-AS1 and their interactions in hepatocellular carcinoma.
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乙型肝炎病毒基因型、ZNRD1-AS1表达数量性状位点及其在肝细胞癌中的相互作用
DOI：
10.18632/oncotarget.9854
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发表时间：
2016-07-12
期刊：
影响因子：
--
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Liu Z;Song C;Wen J;Xu L;Liu Y;Zhu J;Zhu L;Hu Z;Ma H;Liu L
含有锌带结构域1反义RNA 1 （ZNRD1-AS1）的遗传变异已被报道与肝细胞癌（HCC）的发展有关。我们试图确定ZNRD1-AS1多态性及其与乙型肝炎病毒（HBV）基因型的相互作用对HCC风险的影响。在这项研究中，我们进行了一项大型人群病例对照研究，纳入了1507例HBV相关HCC病例和1560例HBV持续性携带者。ZNRD1-AS1基因的3个单核苷酸多态性（rs3757328、rs6940552和rs9261204）采用TaqMan等位基因鉴别法分型，并采用多重PCR法鉴定HBV基因型。我们发现ZNRD1-AS1 rs6940552和rs9261204 snp与HCC风险增加之间存在一致的显著相关性（加性遗传模型：rs6940552校正OR = 1.16, 95% CI = 1.03-1.32； rs9261204校正OR =1.20, 95% CI = 1.06-1.35），并发现rs3757328与HCC风险之间存在临界相关性。此外，我们观察到snp变异等位基因数量的增加与HCC风险之间存在剂量依赖关系（趋势P <0.001）。此外，我们观察到，与HBV b相关基因型（校正OR = 0.89, 95% CI = 0.69-1.15；异质性检验P= 0.029）相比，三种snp对非b基因型HBV感染者HCC风险的综合影响更强（校正OR = 1.26, 95% CI = 1.05-1.50）。我们还发现变异等位基因与HBV基因型之间的增殖相互作用显著影响HCC易感性（P = 0.030）。总之，这些结果表明ZNRD1-AS1可能影响HBV基因型伴发HCC的风险。
Genetic variants in zinc ribbon domain-containing 1 antisense RNA 1 (ZNRD1-AS1) have been reported to be associated with development of hepatocellular carcinoma (HCC). We sought to determine the influences of ZNRD1-AS1 polymorphisms and their interactions with Hepatitis B virus (HBV) genotypes on the risk of HCC. In this study, we conducted a large population case-control study with 1,507 HBV-related HCC cases and 1,560 HBV persistent carriers. Three single-nucleotide polymorphisms (SNPs) in ZNRD1-AS1 (rs3757328, rs6940552 and rs9261204) were genotyped using a TaqMan allelic discrimination assay, and the HBV genotypes were identified by multiplex PCR. We found consistently significant associations between the ZNRD1-AS1 rs6940552 and rs9261204 SNPs with an increased risk of HCC (additive genetic model: adjusted OR = 1.16, 95% CI = 1.03-1.32 for rs6940552; adjusted OR =1.20, 95% CI = 1.06-1.35 for rs9261204) and found a borderline association between rs3757328 and HCC risk. Besides, we observed a dose-dependent relationship between increasing numbers of variant alleles of the SNPs and HCC risk (P for trend <0.001). Moreover, we observed a stronger combined effect of the three SNPs on HCC risk among the subjects infected with non-B genotype HBV (adjusted OR = 1.26, 95% CI = 1.05-1.50) compared with HBV B-related genotypes (adjusted OR = 0.89, 95% CI = 0.69-1.15; P= 0.029 for heterogeneity test). We also found that a multiplicative interaction between the variant alleles and the HBV genotype significantly affected HCC susceptibility (P = 0.030). Together, these results indicate that ZNRD1-AS1 may influence HCC risk accompanied by HBV genotypes.
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DOI：
10.1093/jnci/djn243
发表时间：
2008-08-20
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Yang HI;Yeh SH;Chen PJ;Iloeje UH;Jen CL;Su J;Wang LY;Lu SN;You SL;Chen DS;Liaw YF;Chen CJ;REVEAL-HBV Study Group
通讯作者：
REVEAL-HBV Study Group
影响因子：
13.5
作者：
Yang, Fu;Zhang, Ling;Sun, Shu-han
通讯作者：
Sun, Shu-han
影响因子：
30.8
作者：
Hu, Zhibin;Liu, Yao;Shen, Hongbing
通讯作者：
Shen, Hongbing
影响因子：
29.4
作者：
Kao, JH;Chen, PJ;Chen, DS
通讯作者：
Chen, DS
影响因子：
2.2
作者：
Xi-Lin, Z.;Te, D.;Hui, L.
通讯作者：
Hui, L.