Interpretation of ANA indirect immunofluorescence test outside the darkroom using NOVA view compared to manual microscopy.
Interpretation of ANA indirect immunofluorescence test outside the darkroom using NOVA view compared to manual microscopy.
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DOI：
10.1155/2014/149316
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发表时间：
2014
影响因子：
4.1
通讯作者：
Hill HR
中科院分区：
文献类型：
--
作者：
Copple SS;Jaskowski TD;Giles R;Hill HR
目标。评估NOVA View的重点是阅读存档图像，而不是由经验丰富、经过认证的医疗技术专家对ANA HEp-2载玻片进行基于显微镜的人工解读。方法：对44例类风湿关节炎、50例系统性红斑狼疮、35例硬皮病、19例Sjögren综合征、10例多发性肌炎患者的369例明确的血清和99例健康对照进行检测。此外，还包括12份来自疾病控制中心的确定血清和100份随机送到ARUP实验室进行ANA HEp-2 IIF检测的患者血清。使用NOVA View上的存档图像读取样本，并与手动读取的结果进行比较。结果。在1:40 / 1:80的稀释下，结果比较显示94.8%/92.9%的阳性，97.4%/97.4%的阴性，96.5%/96.2%的手动IIF和NOVA View存档图像之间的总一致性。两种方法之间可识别模式的一致性为97%，PCNA和混合模式尚不确定。结论。在NOVA视图上读取存档图像与在荧光显微镜上手动读取存档图像之间获得了极好的一致性。此外，还观察到工作流的好处，需要在未来的研究中进行分析。
Objective. To evaluate NOVA View with focus on reading archived images versus microscope based manual interpretation of ANA HEp-2 slides by an experienced, certified medical technologist. Methods. 369 well defined sera from: 44 rheumatoid arthritis, 50 systemic lupus erythematosus, 35 scleroderma, 19 Sjögren's syndrome, and 10 polymyositis patients as well as 99 healthy controls were examined. In addition, 12 defined sera from the Centers for Disease Control and 100 random patient sera sent to ARUP Laboratories for ANA HEp-2 IIF testing were included. Samples were read using the archived images on NOVA View and compared to results obtained from manual reading. Results. At a 1 : 40/1 : 80 dilution the resulting comparison demonstrated 94.8%/92.9% positive, 97.4%/97.4% negative, and 96.5%/96.2% total agreements between manual IIF and NOVA View archived images. Agreement of identifiable patterns between methods was 97%, with PCNA and mixed patterns undetermined. Conclusion. Excellent agreements were obtained between reading archived images on NOVA View and manually on a fluorescent microscope. In addition, workflow benefits were observed which need to be analyzed in future studies.
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影响因子：
--
作者：
Willitzki A;Hiemann R;Peters V;Sack U;Schierack P;Rödiger S;Anderer U;Conrad K;Bogdanos DP;Reinhold D;Roggenbuck D
通讯作者：
Roggenbuck D
影响因子：
6.8
作者：
Van Blerk, Marjan;Van Campenhout, Christel;Libeer, Jean-Claude
通讯作者：
Libeer, Jean-Claude
影响因子：
13.6
作者：
Hiemann, Rico;Buettner, Thomas;Conrad, Karsten
通讯作者：
Conrad, Karsten
影响因子：
--
作者：
Voigt J;Krause C;Rohwäder E;Saschenbrecker S;Hahn M;Danckwardt M;Feirer C;Ens K;Fechner K;Barth E;Martinetz T;Stöcker W
通讯作者：
Stöcker W
影响因子：
6.8
作者：
Bonroy, Carolien;Verfaillie, Charlotte;Devreese, Katrien
通讯作者：
Devreese, Katrien