Human intestinal M cells exhibit enterocyte-like intermediate filaments
Human intestinal M cells exhibit enterocyte-like intermediate filaments
复制标题
人肠 M 细胞表现出肠细胞样中间丝
作者：
T. Kucharzik;N. Lügering;K. W. Schmid;M. Schmidt;R. Stoll;Wolfram Domschke
背景：覆盖Peyer氏斑淋巴滤泡的M细胞的来源和超微结构组成尚不清楚。不同动物模型的结果表明，M细胞和邻近肠细胞之间的中间细丝组成存在物种特异性差异。然而，人们对人类M细胞的中间纤维知之甚少。目的：比较人M细胞与相邻吸收性肠细胞的细胞骨架成分。方法采用免疫组化和免疫金电镜技术检测人阑尾滤泡相关上皮M细胞中不同细胞角蛋白、波形蛋白和去蛋白的表达和定位。结果：人肠上皮细胞特异性细胞角蛋白8、18、19和20在吸收型肠细胞和M细胞中均有表达，两种细胞在强度和细胞分布上无差异。肠细胞和M细胞中没有检测到波形蛋白和聚丝蛋白，它们是间充质细胞或肌源性细胞的组织特异性标记物，以及其他细胞角蛋白。结论：首次对人肠道M细胞中间丝的结构进行了研究。我们的研究结果表明，与几种动物模型相比，人类M细胞在中间丝细胞骨架的组成上显然与邻近的肠细胞没有区别。肠细胞样细胞角蛋白的存在和其他细胞角蛋白以及vimentin和desmin的缺失支持了人类肠道M细胞起源于上皮的假设，并提示M细胞可能来源于分化的肠细胞。
Background—The derivation and ultrastructural composition of M cells covering the lymphoid follicles of Peyer’s patches is still unknown. Results from different animal models have shown that there are species specific differences in the composition of intermediate filaments between M cells and neighbouring enterocytes. Little is known, however, about intermediate filaments of human M cells. Aims—To compare components of the cytoskeleton of human M cells with those of adjacent absorptive enterocytes. Methods—The expression and localisation of different cytokeratins, vimentin, and desmin in M cells was determined on follicle associated epithelia of human appendix using immunohistochemistry and immunogold electron microscopy. Results—Cytokeratins specific for human intestinal epithelial cells such as cytokeratins 8, 18, 19, and 20 were expressed in both absorptive enterocytes and M cells with no differences in intensity and cellular distribution between both cell types. Vimentin and desmin, tissue specific markers of either mesenchymal or myogenic cells, as well as other cytokeratins were not detectable in enterocytes or M cells. Conclusion—This is the first study on the structure of intermediate filaments in human intestinal M cells. Our results show that in contrast to several animal models, human M cells apparently do not differ from adjacent enterocytes in the composition of their intermediate filament cytoskeleton. The presence of enterocyte like cytokeratins and the absence of other cytokeratins as well as of vimentin and desmin supports the hypothesis of an epithelial origin of human intestinal M cells and suggests that M cells may derive from differentiated enterocytes.
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肠道 M 细胞的结构和功能。
影响因子：
3.7
作者：
Trier,JS
通讯作者：
Trier,JS
DOI：
10.1152/ajpgi.1994.267.6.g1108
发表时间：
1994-12
期刊：
The American journal of physiology
影响因子：
--
作者：
Paul J. Giannasca;K. T. Giannasca;P. Falk;J. Gordon;Marian R. Neutra
通讯作者：
Paul J. Giannasca;K. T. Giannasca;P. Falk;J. Gordon;Marian R. Neutra
影响因子：
56.9
作者：
WOLF, JL;RUBIN, DH;FIELDS, BN
通讯作者：
FIELDS, BN
肠道相关淋巴组织上皮的粘附性不同。
DOI：
--
发表时间：
1988
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Pappo,J;Steger,HJ;Owen,RL
通讯作者：
Owen,RL
呼肠孤病毒与小鼠肠道肠道 M 细胞和吸收细胞相互作用的决定因素。
影响因子：
29.4
作者：
Wolf,JL;Kauffman,RS;Finberg,R;Dambrauskas,R;Fields,BN;Trier,JS
通讯作者：
Trier,JS