Splice variants of the SIP1 transcripts play a role in nodule organogenesis in Lotus japonicus
Splice variants of the SIP1 transcripts play a role in nodule organogenesis in Lotus japonicus
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SIP1转录本的剪接变体在百脉根结节器官发生中发挥作用
DOI：
10.1007/s11103-013-0042-3
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发表时间：
2013-03
影响因子：
5.1
通讯作者：
Zhongming Zhang
中科院分区：
文献类型：
--
作者：
Longxiang Wang;Heng Kang;Zonglie Hong;Zhongming Zhang
SymRK相互作用蛋白1（SIP 1）先前已被证明与共生受体激酶，SymRK，在莲花。SIP 1转录本SIP 1 L的一个较长的变体被分离和表征。SIP 1 L含有额外的17个氨基酸，使其C-末端成为完整的热休克蛋白20（Hsp 20）样结构域。与SIP 1 S相反，较长的剪接变体SIP 1 L不能与SymRK相互作用。SIP 1 L和SIP 1 S的转录本都可以在发育中的根瘤和其他植物组织中检测到，尽管前者总是比后者更丰富。SIP 1 L和SIP 1 S形成异聚体蛋白复合物，共定位于质膜、细胞质和细胞核中。SIP 1-RNAi在转基因毛状根中的表达导致根瘤和丛枝菌根发育受损，表明SIP 1在共同的共生途径中起重要作用。SIP 1 L或SIP 1 S的过表达增加了转基因毛状根上形成的结节的数量，表明SIP 1在结瘤中的积极作用。在其他高等植物中未检测到SIP 1 S样转录物，这些植物的SIP 1 L样蛋白能够与SymRK同源物相互作用。有人提出，损失的SIP 1 L与SymRK在莲花相互作用的能力是由一个较短的剪接变异体，SIP 1 S，它结合SymRK的表达补偿，并可能发挥作用，在中继共生信号的下游细胞事件。
SymRK-interacting protein 1 (SIP1) has previously been shown to interact with the symbiosis receptor kinase, SymRK, in Lotus japonicus. A longer variant of the SIP1 transcript, SIP1L, was isolated and characterized. SIP1L contains an additional 17 amino acids that make its C-terminus a complete heat shock protein 20 (Hsp20)-like domain. In contrast to SIP1S, the longer splicing variant SIP1L could not interact with SymRK. Both SIP1L and SIP1S transcripts could be detected in developing nodules and other plant tissues, although the former was always more abundant than the latter. SIP1L and SIP1S formed heteromeric protein complexes, which were co-localized in the plasma membrane, cytoplasm and nuclei. Expression of SIP1-RNAi in transgenic hairy roots resulted in impairment in the nodule and arbuscular mycorrhizal development, suggesting an important role of SIP1 in the common symbiosis pathway. Overexpression of either SIP1L or SIP1S increased the number of nodules formed on transgenic hairy roots, indicating a positive role of SIP1 in nodulation. The SIP1S-like transcript was not detected in other higher plants tested, and the SIP1L-like proteins of these plants were capable of interacting with the SymRK orthologs. It is proposed that the loss of the ability of SIP1L to interact with SymRK in Lotus is compensated by the expression of a shorter splicing variant, SIP1S, which binds SymRK and may play a role in relaying the symbiosis signals to downstream cellular events.
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影响因子：
7.4
作者：
G. Oldroyd;S. Long
通讯作者：
G. Oldroyd;S. Long
DOI：
--
发表时间：
2009
期刊：
--
影响因子：
--
作者：
Marc Libault;T. Joshi;V. Benedito;Dong Xu;M. Udvardi;G. Stacey
通讯作者：
Marc Libault;T. Joshi;V. Benedito;Dong Xu;M. Udvardi;G. Stacey
影响因子：
56.9
作者：
Smit, P;Raedts, J;Geurts, R
通讯作者：
Geurts, R
影响因子：
7.4
作者：
R. Wais;D. Keating;S. Long
通讯作者：
R. Wais;D. Keating;S. Long
影响因子：
11.6
作者：
Hirsch, Sibylle;Kim, Jiyoung;Oldroyd, Giles E. D.
通讯作者：
Oldroyd, Giles E. D.