GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.).
GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.).
复制标题
DOI：
10.1038/srep35040
复制
发表时间：
2016-10-07
影响因子：
4.6
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Liang C;Meng Z;Meng Z;Malik W;Yan R;Lwin KM;Lin F;Wang Y;Sun G;Zhou T;Zhu T;Li J;Jin S;Guo S;Zhang R
bZIP转录因子（TF）是脱落酸（阿坝）介导的植物非生物胁迫信号转导途径的重要调节因子。在这里，我们报告了GhABF2的克隆和特性，编码典型的棉花bZIP TF。GhABF2的过表达显著提高了拟南芥和棉花的耐旱性和耐盐性。然而，GhABF2基因的沉默使转基因棉花对PEG渗透胁迫和盐胁迫敏感。干旱和阿坝处理诱导GhABF2的表达，但高盐抑制。转录组分析表明，GhABF2通过调控阿坝、干旱和盐响应相关基因来提高植物的耐旱性和耐盐性。干旱和盐胁迫后，GhABF2基因过表达棉花的脯氨酸含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性均显著高于野生型棉花。干旱和盐碱湿地下纤维产量的增加表明GhABF2在提高转基因株系的耐旱性和耐盐性方面发挥了重要作用。总之，操纵GhABF2的生物技术工具可以是一个可持续的战略部署棉花耐旱耐盐。
The bZIP transcription factor (TF) act as an important regulator for the abscisic acid (ABA) mediated abiotic stresses signaling pathways in plants. Here, we reported the cloning and characterization of GhABF2, encoding for typical cotton bZIP TF. Overexpression of GhABF2 significantly improved drought and salt stress tolerance both in Arabidopsis and cotton. However, silencing of GhABF2 made transgenic cotton sensitive to PEG osmotic and salt stress. Expression of GhABF2 was induced by drought and ABA treatments but repressed by high salinity. Transcriptome analysis indicated that GhABF2 increases drought and salt tolerance by regulating genes related to ABA, drought and salt response. The proline contents, activity of superoxide dismutase (SOD) and catalase (CAT) were also significantly increased in GhABF2-overexpression cottons in comparison to wild type after drought and salt treatment. Further, an increase in fiber yield under drought and saline-alkali wetland exhibited the important role of GhABF2 in enhancing the drought and salt tolerance in transgenic lines. In conclusion, manipulation of GhABF2 by biotechnological tools could be a sustainable strategy to deploy drought and salt tolerance in cotton.
登录
查看更多内容
细胞分裂素生物合成基因 IPT 的缺水诱导表达可提高棉花的耐旱性
影响因子：
3.7
作者：
Kuppu S;Mishra N;Hu R;Sun L;Zhu X;Shen G;Blumwald E;Payton P;Zhang H
通讯作者：
Zhang H
影响因子：
4.4
作者：
Chaudhary B;Hovav R;Flagel L;Mittler R;Wendel JF
通讯作者：
Wendel JF
DOI：
10.1073/pnas.0505667103
发表时间：
2006-02-07
影响因子：
11.1
作者：
Furihata, T;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
46.9
作者：
Li, Fuguang;Fan, Guangyi;Yu, Shuxun
通讯作者：
Yu, Shuxun
DOI：
10.1073/pnas.96.26.15348
发表时间：
1999-12-21
影响因子：
11.1
作者：
Hobo, T;Kowyama, Y;Hattori, T
通讯作者：
Hattori, T