Characterization, using comparative proteomics, of differentially expressed proteins in the hippocampus of the mesial temporal lobe of epileptic rats following treatment with valproate
Characterization, using comparative proteomics, of differentially expressed proteins in the hippocampus of the mesial temporal lobe of epileptic rats following treatment with valproate
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使用比较蛋白质组学表征癫痫大鼠经丙戊酸钠治疗后内侧颞叶海马中差异表达的蛋白质
DOI：
10.1007/s00726-010-0638-8
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发表时间：
2010
期刊：
影响因子：
3.5
通讯作者：
Yin F.
中科院分区：
文献类型：
--
作者：
Wu L;Peng J;Wei C;Liu G;Wang G;Li K;Yin F.
The objective of the study was to explore the pathogenesis of mesial temporal lobe epilepsy (MTLE) and the mechanism of valproate administration in the early stage of MTLE development. We performed a global comparative analysis and function classification of differentially expressed proteins using proteomics. MTLE models of developmental rats were induced by lithium-pilocarpine. Proteins in the hippocampus were separated by 2-DE technology. PDQuest software was used to analyze 2-DE images, and MALDI-TOF-MS was used to identify the differentially expressed proteins. Western blot was used to determine the differential expression levels of synapse-related proteins synapsin-1, dynamin-1 and neurogranin in both MTLE rat and human hippocampus. A total of 48 differentially expressed proteins were identified between spontaneous and non-spontaneous MTLE rats, while 41 proteins between MTLE rats and post valproate-treatment rats were identified. All of the proteins can be categorized into several groups by biological functions: synaptic and neurotransmitter release, cytoskeletal structure and dynamics, cell junctions, energy metabolism and mitochondrial function, molecular chaperones, signal regulation and others. Western blot results were similar to the changes noted in 2-DE. The differentially expressed proteins, especially the proteins related to synaptic and neurotransmitter release function, such as synapsin-1, dynamin-1 and neurogranin, are probably involved in the mechanism of MTLE and the pharmacological effect of valproate. These findings may provide important clues to elucidate the mechanism of chronic MTLE and to identify an optimum medication intervention time and new biomarkers for the development of pharmacological therapies targeted at epilepsy.
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影响因子：
5.3
作者：
Demand, J;Lüders, J;Höhfeld, J
通讯作者：
Höhfeld, J
影响因子：
5.6
作者：
P. Williams;P. Dou;F. Dudek
通讯作者：
P. Williams;P. Dou;F. Dudek
离子通道与癫痫
影响因子：
2.3
作者：
M. Mazzuca;F. Lesage;M. Lazdunski
通讯作者：
M. Lazdunski
影响因子：
6.1
作者：
E. Schuller;T. Gulesserian;R. Seidl;N. Cairns;G. Lube
通讯作者：
E. Schuller;T. Gulesserian;R. Seidl;N. Cairns;G. Lube
DOI：
10.1016/s0168-0102(98)81944-5
发表时间：
1998-12
期刊：
The Journal of Cell Biology
影响因子：
--
作者：
S. Terada;T. Tsujimoto;Y. Takei;Tomoyuki Takahashi;N. Hirokawa
通讯作者：
S. Terada;T. Tsujimoto;Y. Takei;Tomoyuki Takahashi;N. Hirokawa