Gold-based nanoparticles realize photothermal and photodynamic synergistic treatment of liver cancer and improve the anaerobic tumor microenvironment under near-infrared light.
Gold-based nanoparticles realize photothermal and photodynamic synergistic treatment of liver cancer and improve the anaerobic tumor microenvironment under near-infrared light.
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金基纳米粒子实现光热光动力协同治疗肝癌并改善近红外光下厌氧肿瘤微环境
DOI：
10.3389/fbioe.2022.957349
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发表时间：
2022
影响因子：
5.7
通讯作者：
You, Zhen
中科院分区：
文献类型：
--
作者：
Li, Bei;Fu, Yi;Xie, Maodi;Feng, Lei;Niu, Xiaoya;Que, Lin;You, Zhen
为了解决手术治疗等传统癌症治疗方法带来的不同痛苦，纳米给药系统为癌症治疗提供了新思路。本文制备了一种新型抗肿瘤治疗纳米粒子P(AAm-co-AN)-AuNRs@CeO2-Ce6(PA/Ce6)，为肝癌的治疗提供了新思路。将涂覆CeO2的金纳米棒接枝到温敏聚合物P(AAm-co-AN)-CTPD的表面。光敏剂Ce6负载在纳米颗粒和聚合物层的表面。 CeO2可以有效缓解肿瘤厌氧微环境，在808 nm近红外（NIR）激发下，金纳米棒实现光热转换，引起局部加热，从而导致聚合物层发生相变，实现可控释放机制。此外，660 nm近红外光可以有效诱导Ce6产生单线态氧，从而有效杀死癌细胞。在 808 nm 激光照射下 600 秒内，PA/Ce6 溶液可加热至约 60°C，足以消融癌细胞和肿瘤组织。当温度为50℃时，Ce6的累积释放率为95.31%。在808 nm激光照射下，PA/Ce6的产氧能力较高，可以有效降低癌细胞中过氧化氢的含量。与游离 Ce6 相比，PA/Ce6 纳米粒子的活性氧介导的荧光更强。 660 nm 和 880 nm 激光同时照射后，HepG2 细胞的细胞活力和迁移能力下降。癌细胞被进一步抑制，表现出良好的体外抗肿瘤作用。 PA-DOX在近红外激光照射下表现出最好的肿瘤生长抑制作用，并且在体内无急性毒性。由于AuNRs的存在，纳米粒子具有高效的光热转换能力，实现光热治疗。 Ce6在660 nm激光的激发下可以产生单线态氧，实现光动力治疗。实验结果还表明，PA/Ce6在激光照射下可以有效分解过氧化氢，旨在有效缓解肿瘤的厌氧微环境。这些表明PA/Ce6在肝细胞癌的治疗中具有广阔的前景。
In order to solve the different pains caused by traditional cancer treatment methods such as surgical treatment, the nano-drug delivery system provides new ideas for cancer treatment. In this paper, a novel anti-tumor therapy nanoparticle, P(AAm-co-AN)-AuNRs@CeO2-Ce6(PA/Ce6), is prepared, which provides a novel idea for liver cancer treatment. The CeO2-coated gold nanorods were grafted onto the surface of the temperature-sensitive polymer P(AAm-co-AN)-CTPD. The photosensitizer Ce6 is loaded on the surface of the nanoparticles and the polymer layer. CeO2 can effectively alleviate the tumor anaerobic microenvironment, and under 808 nm near-infrared (NIR) excitation, the gold nanorods achieve photothermal conversion to induce local heating, which leads to the phase transition of the polymer layer and realizes a controllable release mechanism. In addition, 660 nm NIR light can effectively induce Ce6 to produce singlet oxygen, thereby effectively killing cancer cells. Under the 808 nm laser irradiation within 600 s, the PA/Ce6 solution can heat up to about 60°C, which was enough to ablate both cancer cells and tumor tissues. When the temperature was 50°C, the cumulative release rate of Ce6 was 95.31%. Under the 808 nm laser irradiation, oxygen production capacity of PA/Ce6 was higher and can effectively reduce the content of hydrogen peroxide in cancer cells. Compared to free Ce6, the reactive oxygen species-mediated fluorescence of PA/Ce6 nanoparticles was greater. The cell viability and migration of HepG2 cells were decreased after the 660 and 880 nm lasers were irradiated at the same time. The cancer cells were further inhibited, showing a good in vitro anti-tumor effect. PA-DOX showed the best tumor growth inhibitory effect under NIR laser irradiation and had no acute toxicity in vivo. Due to the existence of AuNRs, nanoparticles had high-efficiency photothermal conversion ability to achieve photothermal therapy. Ce6 can generate singlet oxygen under the excitation of 660 nm laser to realize photodynamic therapy. The experimental results also showed that PA/Ce6 can effectively decompose hydrogen peroxide under laser irradiation, aiming to effectively alleviate the anaerobic microenvironment of tumors. These indicate that PA/Ce6 plays a promising role for hepatocellular carcinoma treatment.
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DOI：
10.1073/pnas.1619302114
发表时间：
2017-04-11
影响因子：
11.1
作者：
Ali, Moustafa R. K.;Rahman, Mohammad Aminur;El-Sayed, Mostafa A.
通讯作者：
El-Sayed, Mostafa A.
影响因子：
19
作者：
Manzano, Miguel;Vallet-Regi, Maria
通讯作者：
Vallet-Regi, Maria
通过具有上临界溶液温度嵌段共聚物和金纳米棒的混合纳米平台进行近红外激活的“关/开”光动力疗法
影响因子：
6.2
作者：
Huang, Baoxuan;Tian, Jia;Zhang, Weian
通讯作者：
Zhang, Weian
影响因子：
5.5
作者：
Hwang, Hee Sook;Shin, Heejun;Na, Kun
通讯作者：
Na, Kun
影响因子：
3.3
作者：
Lee, Yu-Chi;Wang, Jing-Houng;Kuo, Yuan-Hung
通讯作者：
Kuo, Yuan-Hung