SARS-CoV-2 infection elicits a rapid neutralizing antibody response that correlates with disease severity.
SARS-CoV-2 infection elicits a rapid neutralizing antibody response that correlates with disease severity.
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DOI：
10.1038/s41598-021-81862-9
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发表时间：
2021-01-28
影响因子：
4.6
通讯作者：
Blanco J
中科院分区：
文献类型：
--
作者：
Trinité B;Tarrés-Freixas F;Rodon J;Pradenas E;Urrea V;Marfil S;Rodríguez de la Concepción ML;Ávila-Nieto C;Aguilar-Gurrieri C;Barajas A;Ortiz R;Paredes R;Mateu L;Valencia A;Guallar V;Ruiz L;Grau E;Massanella M;Puig J;Chamorro A;Izquierdo-Useros N;Segalés J;Clotet B;Carrillo J;Vergara-Alert J;Blanco J
中和抗体对SARS-CoV-2感染者的保护作用尚未明确。为了解决这个问题，我们分析了中和抗体反应的动力学及其与疾病严重程度的关系。在2020年3月至5月期间，前瞻性KING研究招募了72名COVID-19+参与者，根据疾病严重程度进行分组。经血清学和病毒学检测确诊为SARS-CoV-2感染。针对复制型病毒和假病毒颗粒评估血浆中和应答。采用多元回归和非参数检验分析参数的相关性。中和滴度的大小随疾病严重程度显著增加。与轻度症状和无症状个体相比，住院个体的滴度更高，两者在50%的病例中共同显示低于检测限的滴度。纵向分析证实了非住院和住院参与者之间的中和滴度存在很大差异，并显示住院患者中中和抗体出现的快速动力学（症状发作后11天和17天分别达到最大活性的50%和80%）。在该有限队列中未观察到年龄、性别或治疗对中和滴度的显著影响。这些数据确定了体液免疫与疾病严重程度的明确关联，并指出抗体以外的免疫机制是COVID-19保护的相关参与者。
The protective effect of neutralizing antibodies in SARS-CoV-2 infected individuals is not yet well defined. To address this issue, we have analyzed the kinetics of neutralizing antibody responses and their association with disease severity. Between March and May 2020, the prospective KING study enrolled 72 COVID-19+ participants grouped according to disease severity. SARS-CoV-2 infection was diagnosed by serological and virological tests. Plasma neutralizing responses were assessed against replicative virus and pseudoviral particles. Multiple regression and non-parametric tests were used to analyze dependence of parameters. The magnitude of neutralizing titers significantly increased with disease severity. Hospitalized individuals developed higher titers compared to mild-symptomatic and asymptomatic individuals, which together showed titers below the detection limit in 50% of cases. Longitudinal analysis confirmed the strong differences in neutralizing titers between non-hospitalized and hospitalized participants and showed rapid kinetics of appearance of neutralizing antibodies (50% and 80% of maximal activity reached after 11 and 17 days after symptoms onset, respectively) in hospitalized patients. No significant impact of age, gender or treatment on the neutralizing titers was observed in this limited cohort. These data identify a clear association of humoral immunity with disease severity and point to immune mechanisms other than antibodies as relevant players in COVID-19 protection.
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