Cellular proliferation in the vitreous: the use of vitreous explants as a model system.
Cellular proliferation in the vitreous: the use of vitreous explants as a model system.
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玻璃体中的细胞增殖：使用玻璃体外植体作为模型系统。
DOI：
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发表时间：
1986
影响因子：
4.4
通讯作者：
J. Lackie
中科院分区：
文献类型：
--
作者：
J. Forrester;R. Docherty;C. Kerr;J. Lackie
使用牛玻璃体凝胶外植体建立了玻璃体中细胞增殖的体外模型。将各种细胞类型，包括鸡胚色素视网膜上皮细胞、脉络膜成纤维细胞、视网膜神经胶质细胞、牛视网膜毛细血管内皮细胞和腹膜小鼠巨噬细胞，在37 ℃下在玻璃体凝胶上培养长达8天，并比较它们对凝胶结构的影响。脉络膜成纤维细胞导致凝胶尺寸非常显着减小，并对凝胶原纤维结构产生最强的牵引力;相比之下，腹膜巨噬细胞几乎没有导致玻璃体体积减少，并且相差显微镜检测到对凝胶的牵引力很小或没有可见的牵引力。剩余的细胞类型通常在凝胶表面形成薄片;这与玻璃体积约50%的初始减少有关，但此后几乎没有变化。细胞介导的玻璃体凝胶的结构上的牵引事件之后，通过延时视频显微镜和电子显微镜在凝胶上接种细胞后的不同时间。这些表现与玻璃体细胞增殖的临床病理学研究密切相关。我们认为，这种体外模型具有几个优点，在体内模型的细胞增殖的玻璃体中，因为它允许分析单个细胞的行为，它是非常适合药理学操作。
An in vitro model of cellular proliferation in the vitreous has been developed using explants of bovine vitreous gel. Various cell types, including chick embryo pigmented retinal epithelium, choroidal fibroblasts, retinal glial cells, bovine retinal capillary endothelial cells, and peritoneal mouse macrophages, were cultured at 37 degrees C on vitreous gels for periods up to 8 days and compared for their effects on the structure of the gel. Choroidal fibroblasts caused a very marked reduction in the size of the gel and produced the strongest traction on the gel fibril structure; in contrast, peritoneal macrophages caused virtually no reduction in vitreous volume and little or no visible traction on the gel as detected by phase-contrast microscopy. The remaining cell types usually formed sheets on the surface of the gel; this was associated with an initial reduction in vitreous volume of approximately 50%, but little change thereafter. The cell mediated traction events on the structure of the vitreous gel were followed by time lapse video microscopy and by electron microscopy at various times after seeding of cells on the gels. The appearances corresponded closely with reported clinico-pathological studies of cellular proliferation in the vitreous. We believe that this in vitro model has several advantages over in vivo models of cellular proliferation in the vitreous, in that it permits analysis of individual cell behaviour and it is eminently suitable for pharmacologic manipulation.
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视网膜血管内皮细胞和周细胞。
影响因子：
4.4
作者：
Buzney,SM;Massicotte,SJ;Hetu,N;Zetter,BR
通讯作者：
Zetter,BR
影响因子：
3.7
作者：
RICHARDS, J;PASCO, D;NANDI, S
通讯作者：
NANDI, S
影响因子：
4.2
作者：
N. Radtke;Yasuo Tano;David B. Chandler;R. Machemer
通讯作者：
N. Radtke;Yasuo Tano;David B. Chandler;R. Machemer
DOI：
10.1001/archopht.1981.03930010869016
发表时间：
1981
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Ussmann,JH;Lazarides,E;Ryan,SJ
通讯作者：
Ryan,SJ
DOI：
10.1007/bf02171726
发表时间：
1983
期刊：
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
影响因子：
--
作者：
Trese,M;Chandler,DB;Machemer,R
通讯作者：
Machemer,R