TAT and HA2 facilitate cellular uptake of gold nanoparticles but do not lead to cytosolic localisation.
TAT and HA2 facilitate cellular uptake of gold nanoparticles but do not lead to cytosolic localisation.
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DOI：
10.1371/journal.pone.0121683
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Lévy R
中科院分区：
文献类型：
--
作者：
Cesbron Y;Shaheen U;Free P;Lévy R
目前可用于将功能标签和药物传递到细胞细胞质的方法效率低下，这构成了细胞生物学（传感器和成像探针的传递）和治疗（药物进入细胞内部机制）的主要障碍。由于细胞膜对大多数分子货物是不可渗透的，病毒肽已被用于通过内吞作用加强其内化，并通过破坏内体囊泡帮助其释放到细胞质中。然而，关于胞质递送的程度，报道了相互矛盾的结果。为了评估它们的潜力，我们使用金纳米颗粒作为模型货物，并系统地评估了病毒肽TAT和HA2或两者对其表面的功能化如何影响其细胞内递送。我们利用光热显微镜评估了细胞内化后存在的金纳米颗粒的数量，并利用电子显微镜评估了它们的亚细胞定位。虽然当TAT和/或HA2病毒肽存在于其表面时，它们的摄取增加，但我们没有观察到金纳米颗粒的显著细胞质递送。
The methods currently available to deliver functional labels and drugs to the cell cytosol are inefficient and this constitutes a major obstacle to cell biology (delivery of sensors and imaging probes) and therapy (drug access to the cell internal machinery). As cell membranes are impermeable to most molecular cargos, viral peptides have been used to bolster their internalisation through endocytosis and help their release to the cytosol by bursting the endosomal vesicles. However, conflicting results have been reported on the extent of the cytosolic delivery achieved. To evaluate their potential, we used gold nanoparticles as model cargos and systematically assessed how the functionalisation of their surface by either or both of the viral peptides TAT and HA2 influenced their intracellular delivery. We evaluated the number of gold nanoparticles present in cells after internalisation using photothermal microscopy and their subcellular localisation by electron microscopy. While their uptake increased when the TAT and/or HA2 viral peptides were present on their surface, we did not observe a significant cytosolic delivery of the gold nanoparticles.
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影响因子：
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