Trim9 regulates activity-dependent fine-scale topography in Drosophila.
Trim9 regulates activity-dependent fine-scale topography in Drosophila.
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Trim9 调节果蝇活动依赖性精细尺度地形
DOI：
10.1016/j.cub.2014.03.041
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发表时间：
2014-05-05
期刊：
影响因子：
9.2
通讯作者：
Ye, Bing
中科院分区：
文献类型：
--
作者：
Yang, Limin;Li, Ruonan;Kaneko, Thkuya;Takle, Kendra;Morikawa, Rei K.;Essex, Laura;Wang, Xin;Zhou, Jie;Emoto, Kazuo;Xiang, Yang;Ye, Bing
传入终末在中枢神经系统(CNS)的二维地图上的地形图投影是神经系统用来编码感觉刺激位置的一般策略。在脊椎动物中，众所周知，虽然引导线索对于建立粗略的地形图至关重要，但神经活动指导着相邻传入终末之间的精细地形图。然而，精细地形的活性依赖调控背后的分子机制却知之甚少。对相邻传入终末之间空间关系的分子分析需要对单个神经元的突触前终末进行可靠的定位，以及体内单细胞分辨率的遗传操作。虽然这两个要求在果蝇身上都有可能满足，但在果蝇中还没有发现依赖活动的地形系统。在这里，我们报告了一个由果蝇神经元活动形成的地形图系统。通过这个系统，我们发现相邻伤害性神经元突触前终末的地形分离需要不同水平的Trim9，一种进化上保守的信号分子。神经活动调节Trim9蛋白水平以指导感觉传入的精细地形图。这项研究既提供了一种神经活动指导轴突终末精细地形图的新机制，也提供了一种在单神经元分辨率下研究这一过程的新系统。
Topographic projection of afferent terminals into two-dimensional maps in the central nervous system (CNS) is a general strategy used by the nervous system to encode the locations of sensory stimuli. In vertebrates, it is known that while guidance cues are critical for establishing a coarse topographic map, neural activity directs fine-scale topography between adjacent afferent terminals. However, the molecular mechanism underlying activity-dependent regulation of fine-scale topography is poorly understood. Molecular analysis of the spatial relationship between adjacent afferent terminals requires reliable localization of the presynaptic terminals of single neurons as well as genetic manipulations with single-cell resolution in vivo. Although both requirements can potentially be met in Drosophila melanogaster, no activity-dependent topographic system has been identified in flies. Here we report a topographic system that is shaped by neuronal activity in Drosophila. With this system, we found that topographic separation of the presynaptic terminals of adjacent nociceptive neurons requires different levels of Trim9, an evolutionarily conserved signaling molecule. Neural activity regulates Trim9 protein levels to direct fine-scale topography of sensory afferents. This study offers both a novel mechanism by which neural activity directs fine-scale topography of axon terminals and a new system to study this process at single-neuron resolution.
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影响因子：
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