Msl2 is a novel component of the vertebrate DNA damage response.
Msl2 is a novel component of the vertebrate DNA damage response.
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DOI：
10.1371/journal.pone.0068549
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Rea S
中科院分区：
文献类型：
--
作者：
Lai Z;Moravcová S;Canitrot Y;Andrzejewski LP;Walshe DM;Rea S
hMSL2 (male-specific lethal 2, human) is a RING finger protein with ubiquitin ligase activity. Although it has been shown to target histone H2B at lysine 34 and p53 at lysine 351, suggesting roles in transcription regulation and apoptosis, its function in these and other processes remains poorly defined. To further characterize this protein, we have disrupted the Msl2 gene in chicken DT40 cells. Msl2−/− cells are viable, with minor growth defects. Biochemical analysis of the chromatin in these cells revealed aberrations in the levels of several histone modifications involved in DNA damage response pathways. DNA repair assays show that both Msl2−/− chicken cells and hMSL2-depleted human cells have defects in non-homologous end joining (NHEJ) repair. DNA damage assays also demonstrate that both Msl2 and hMSL2 proteins are modified and stabilized shortly after induction of DNA damage. Moreover, hMSL2 mediates modification, presumably ubiquitylation, of a key DNA repair mediator 53BP1 at lysine 1690. Similarly, hMSL1 and hMOF (males absent on the first) are modified in the presence of hMSL2 shortly after DNA damage. These data identify a novel role for Msl2/hMSL2 in the cellular response to DNA damage. The kinetics of its stabilization suggests a function early in the NHEJ repair pathway. Moreover, Msl2 plays a role in maintaining normal histone modification profiles, which may also contribute to the DNA damage response.
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影响因子：
16
作者：
Huen, Michael S. Y.;Huang, Jun;Leung, Justin W. C.;Sy, Shirley M. -H.;Leung, Ka Man;Ching, Yick-Pang;Tsao, Sai Wah;Chen, Junjie
通讯作者：
Chen, Junjie
影响因子：
--
作者：
Brandsma I;Gent DC
通讯作者：
Gent DC
影响因子：
1.6
作者：
Mendjan, Sascha;Akhtar, Asifa
通讯作者：
Akhtar, Asifa
影响因子：
4.8
作者：
Fukushima, T;Takata, M;Takeda, S
通讯作者：
Takeda, S
影响因子：
5.3
作者：
Li, Xiangzhi;Corsa, Callie Ann Sprunger;Dou, Yali
通讯作者：
Dou, Yali