Evolution of an expanded mannose receptor gene family.
Evolution of an expanded mannose receptor gene family.
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DOI：
10.1371/journal.pone.0110330
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Butter C
中科院分区：
文献类型：
--
作者：
Staines K;Hunt LG;Young JR;Butter C
来自一种蛋白质的肽序列，该蛋白质被一种识别鸡巨噬细胞的抗体KUL 01特异性免疫沉淀，鉴定出哺乳动物甘露糖受体MRC 1的同源物，我们称之为MRC 1 L-B。对鸡基因的基因组环境的检查揭示了五个旁系同源基因的阵列，MRC 1 L-A到MRC 1 L-E，位于哺乳动物中单个MRC 1基因两侧的保守侧翼基因之间。在来自巨噬细胞系的RNA和其他RNA中检测到所有五个基因的转录物，其序列允许精确定义剪接的外显子，确认或校正现有的生物信息学注释。已确认的基因结构被用于在另外两种鸟类和彩龟的基因组中定位所有五个基因的直系同源物，所有这些基因都具有完整的编码序列。蜥蜴基因组只有三个基因，一个是MRC 1 L-A的直系同源物，两个是最近重复产生的MRC 1 L-B抗原基因的直系同源物。与大多数哺乳动物一样，非洲爪蟾的基因组在相应的位点上只有一个类似于MRC 1的基因。MRC 1 L-A和MRC 1 L-B基因具有相似的细胞质区域，这可能表明相似的亚细胞迁移和功能。其他三个基因的细胞质区域是非常不同的，可能表明一个新的功能库的进化，这一系列的分子，其中可能包括新的相互作用与病原体。
Sequences of peptides from a protein specifically immunoprecipitated by an antibody, KUL01, that recognises chicken macrophages, identified a homologue of the mammalian mannose receptor, MRC1, which we called MRC1L-B. Inspection of the genomic environment of the chicken gene revealed an array of five paralogous genes, MRC1L-A to MRC1L-E, located between conserved flanking genes found either side of the single MRC1 gene in mammals. Transcripts of all five genes were detected in RNA from a macrophage cell line and other RNAs, whose sequences allowed the precise definition of spliced exons, confirming or correcting existing bioinformatic annotation. The confirmed gene structures were used to locate orthologues of all five genes in the genomes of two other avian species and of the painted turtle, all with intact coding sequences. The lizard genome had only three genes, one orthologue of MRC1L-A and two orthologues of the MRC1L-B antigen gene resulting from a recent duplication. The Xenopus genome, like that of most mammals, had only a single MRC1-like gene at the corresponding locus. MRC1L-A and MRC1L-B genes had similar cytoplasmic regions that may be indicative of similar subcellular migration and functions. Cytoplasmic regions of the other three genes were very divergent, possibly indicating the evolution of a new functional repertoire for this family of molecules, which might include novel interactions with pathogens.
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系统基因分析支持海龟作为姊妹鸟类和鳄鱼（Archosauria）的位置。
影响因子：
5.4
作者：
Chiari Y;Cahais V;Galtier N;Delsuc F
通讯作者：
Delsuc F
影响因子：
5.4
作者：
Reading, PC;Miller, JL;Anders, EM
通讯作者：
Anders, EM
影响因子：
1.8
作者：
Mast, J;Goddeeris, BM;Berghman, LR
通讯作者：
Berghman, LR
影响因子：
32.4
作者：
Prigozy, TI;Sieling, PA;Kronenberg, M
通讯作者：
Kronenberg, M
DOI：
10.1084/jem.182.2.389
发表时间：
1995-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Sallusto F;Cella M;Danieli C;Lanzavecchia A
通讯作者：
Lanzavecchia A