Genetic relatedness of infecting and reinfecting respiratory syncytial virus strains identified in a birth cohort from rural Kenya.
Genetic relatedness of infecting and reinfecting respiratory syncytial virus strains identified in a birth cohort from rural Kenya.
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在肯尼亚农村的出生队列中鉴定出感染和恢复呼吸道合胞病毒菌株的遗传相关性。
DOI：
10.1093/infdis/jis570
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发表时间：
2012-11-15
期刊：
影响因子：
--
通讯作者：
Nokes DJ
中科院分区：
文献类型：
--
作者：
Agoti CN;Mwihuri AG;Sande CJ;Onyango CO;Medley GF;Cane PA;Nokes DJ
背景：呼吸道合胞病毒（RSV）反复感染个体。 这在多大程度上是RSV抗原多样性的结果尚不清楚。方法：对来自肯尼亚农村的635名儿童从出生到连续3次RSV流行期间的RSV感染进行密切监测。 通过对急性呼吸道疾病期间收集的鼻冲洗样本进行免疫荧光检测，确定RSV感染，分型为A组和B组，并在附着（G）蛋白中进行测序。将与先前阳性样本间隔≥14天的阳性样本定义为先验再感染。结果：对409例确诊感染中的325例（80%）进行了系统发育分析，包括83例再感染中的53例（64%）。 在28次发作中观察到异源组再感染，在25次发作中观察到同源组再感染; 10次涉及同源基因型，5次未显示氨基酸变化，3次间隔21-24天，可能是持续性感染。再感染者基因型的时间分布与单次感染者没有差异。结论：绝大多数感染和再感染对在组、基因型或G氨基酸序列（即由不同的病毒组成）上存在差异。 这在多大程度上是感染史或流行多样性的免疫记忆的结果仍不清楚。
Background. Respiratory syncytial virus (RSV) reinfects individuals repeatedly. The extent to which this is a consequence of RSV antigenic diversity is unclear. Methods. Six-hundred thirty-five children from rural Kenya were closely monitored for RSV infection from birth through 3 consecutive RSV epidemics. RSV infections were identified by immunofluorescence testing of nasal washing samples collected during acute respiratory illnesses, typed into group A and B, and sequenced in the attachment (G) protein. A positive sample separated from a previous positive by ≥14 days was defined as a reinfection a priori. Results. Phylogenetic analysis was undertaken for 325 (80%) of 409 identified infections, including 53 (64%) of 83 reinfections. Heterologous group reinfections were observed in 28 episodes, and homologous group reinfections were observed in 25 episodes; 10 involved homologous genotypes, 5 showed no amino acid changes, and 3 were separated by 21–24 days and were potentially persistent infections. The temporal distribution of genotypes among reinfections did not differ from that of single infections. Conclusions. The vast majority of infection and reinfection pairs differed by group, genotype, or G amino acid sequence (ie, comprised distinct viruses). The extent to which this is a consequence of immune memory of infection history or prevalent diversity remains unclear.
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影响因子：
158.5
作者：
HALL, CB;POWELL, KR;COHEN, HJ
通讯作者：
COHEN, HJ
影响因子：
6.4
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HALL, CB;WALSH, EE;SCHNABEL, KC
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SCHNABEL, KC
影响因子：
5.1
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HALL, CB;DOUGLAS, RG;GEIMAN, JM
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影响因子：
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影响因子：
10.7
作者：
Posada, David
通讯作者：
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