Severity of Systemic Inflammatory Response Syndrome Affects the Blood Levels of Circulating Inflammatory-Relevant MicroRNAs.
Severity of Systemic Inflammatory Response Syndrome Affects the Blood Levels of Circulating Inflammatory-Relevant MicroRNAs.
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全身炎症反应综合征的严重程度影响循环中炎症相关微小RNA的血液水平。
DOI：
10.3389/fimmu.2017.01977
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发表时间：
2017
影响因子：
7.3
通讯作者：
Llewelyn MJ
中科院分区：
文献类型：
--
作者：
Caserta S;Mengozzi M;Kern F;Newbury SF;Ghezzi P;Llewelyn MJ
全身炎症反应综合征（SIRS）是由多种形式的组织损伤和感染引发的潜在致死性反应。当全身性炎症由感染引发时，使用术语败血症。了解炎症是如何介导和调节的具有巨大的医学意义。我们先前证明了循环炎症相关microRNA（CIR-miRNA）是区分脓毒症和SIRS的候选生物标志物。在这里，我们着手确定CIR-miRNA水平如何反映SIRS的严重程度以及它们是否来自活化的免疫细胞。临床疾病严重程度评分和红细胞（RBC）损伤或免疫细胞活化标志物与SIRS和脓毒症患者的CIR-miRNA水平相关。在免疫细胞培养物中评估SIRS期间调节的CIR-miRNA的释放。我们发现，非感染性SIRS的严重程度反映在miR-378 a-3 p、miR-30 a-5 p、miR-30 d-5 p和miR-192- 5 p的水平上，而不是脓毒症。这些CIR-miRNA水平与氧化还原生物标志物Peroxiredoxin-1（Prdx-1）的水平呈正相关，该生物标志物先前已被证明在炎症期间由免疫细胞释放。此外，体外活化的免疫细胞产生SIRS相关的miR-378 a-3 p、miR-30 a-5 p、miR-30 d-5 p和miR-192- 5 p。我们的研究进一步了解了CIR-miRNA作为炎症潜在调节因子的起源、作用和运输。
The systemic inflammatory response syndrome (SIRS) is a potentially lethal response triggered by diverse forms of tissue injury and infection. When systemic inflammation is triggered by infection, the term sepsis is used. Understanding how inflammation is mediated and regulated is of enormous medical importance. We previously demonstrated that circulating inflammatory-relevant microRNAs (CIR-miRNAs) are candidate biomarkers for differentiating sepsis from SIRS. Here, we set out to determine how CIR-miRNA levels reflect SIRS severity and whether they derive from activated immune cells. Clinical disease severity scores and markers of red blood cell (RBC) damage or immune cell activation were correlated with CIR-miRNA levels in patients with SIRS and sepsis. The release of CIR-miRNAs modulated during SIRS was assessed in immune cell cultures. We show that severity of non-infective SIRS, but not sepsis is reflected in the levels of miR-378a-3p, miR-30a-5p, miR-30d-5p, and miR-192-5p. These CIR-miRNA levels positively correlate with levels of the redox biomarker, peroxiredoxin-1 (Prdx-1), which has previously been shown to be released by immune cells during inflammation. Furthermore, in vitro activated immune cells produce SIRS-associated miR-378a-3p, miR-30a-5p, miR-30d-5p, and miR-192-5p. Our study furthers the understanding of the origin, role, and trafficking of CIR-miRNAs as potential regulators of inflammation.
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影响因子：
4.4
作者：
Ferguson, KL;Taheri, P;Dechert, R
通讯作者：
Dechert, R
影响因子：
7.3
作者：
Correia CN;Nalpas NC;McLoughlin KE;Browne JA;Gordon SV;MacHugh DE;Shaughnessy RG
通讯作者：
Shaughnessy RG
影响因子：
4.6
作者：
Caserta S;Kern F;Cohen J;Drage S;Newbury SF;Llewelyn MJ
通讯作者：
Llewelyn MJ
DOI：
10.1073/pnas.1209414109
发表时间：
2012-07-31
影响因子：
11.1
作者：
Fabbri, Muller;Paone, Alessio;Croce, Carlo M.
通讯作者：
Croce, Carlo M.
DOI：
10.1007/978-1-4939-3344-0_16
发表时间：
2016-01-01
期刊：
SUPERANTIGENS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Caserta, Stefano;Taylor, Amanda L.;Llewelyn, Martin J.
通讯作者：
Llewelyn, Martin J.