Hyperspectral and chlorophyll fluorescence imaging to analyse the impact of Fusarium culmorum on the photosynthetic integrity of infected wheat ears.
Hyperspectral and chlorophyll fluorescence imaging to analyse the impact of Fusarium culmorum on the photosynthetic integrity of infected wheat ears.
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DOI：
10.3390/s110403765
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Herppich WB
中科院分区：
文献类型：
--
作者：
Bauriegel E;Giebel A;Herppich WB
小麦赤霉病，由镰刀菌属引起，由于在受感染的谷物中同时产生高毒性真菌毒素，因此对农民和粮食生产都是一个严重的问题。对于选择性真菌毒素分析，有关现场感染状况的信息将是有帮助的。直接在穗上进行早期症状检测以及相应的地理位置对于选择性收获非常重要。因此，测试了各种数字成像方法检测冬小麦赤霉病的能力。用实验室高光谱成像系统（波长范围：400 nm至1，000 nm）记录健康和人工镰刀菌感染耳朵的时间序列图像。用成像软件评价疾病特异性光谱特征。应用“光谱角映射器”方法，健康和感染的耳组织可以清楚地分类。同时，进行健康和感染耳朵的叶绿素荧光成像以及疾病严重程度的视觉评级。人工接种后6 ~ 11 d，感病穗的光合效率较健康穗下降。病害严重程度与光合效率高度相关。超过5%的疾病严重程度的感染限制，叶绿素荧光成像可靠地识别感染的耳朵。用这种技术，疾病的严重程度的分化是成功的，在10%的步骤。根据所选择的感兴趣的区域的质量，高光谱成像很容易检测到接种后7天的头疫病高达50%的疾病的严重程度。开始成熟后，健康和患病的耳朵很难区分的评价方法。
Head blight on wheat, caused by Fusarium spp., is a serious problem for both farmers and food production due to the concomitant production of highly toxic mycotoxins in infected cereals. For selective mycotoxin analyses, information about the on-field status of infestation would be helpful. Early symptom detection directly on ears, together with the corresponding geographic position, would be important for selective harvesting. Hence, the capabilities of various digital imaging methods to detect head blight disease on winter wheat were tested. Time series of images of healthy and artificially Fusarium-infected ears were recorded with a laboratory hyperspectral imaging system (wavelength range: 400 nm to 1,000 nm). Disease-specific spectral signatures were evaluated with an imaging software. Applying the ‘Spectral Angle Mapper’ method, healthy and infected ear tissue could be clearly classified. Simultaneously, chlorophyll fluorescence imaging of healthy and infected ears, and visual rating of the severity of disease was performed. Between six and eleven days after artificial inoculation, photosynthetic efficiency of infected compared to healthy ears decreased. The severity of disease highly correlated with photosynthetic efficiency. Above an infection limit of 5% severity of disease, chlorophyll fluorescence imaging reliably recognised infected ears. With this technique, differentiation of the severity of disease was successful in steps of 10%. Depending on the quality of chosen regions of interests, hyperspectral imaging readily detects head blight 7 d after inoculation up to a severity of disease of 50%. After beginning of ripening, healthy and diseased ears were hardly distinguishable with the evaluated methods.
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影响因子：
--
作者：
Kang, ZS;Buchenauer, H
通讯作者：
Buchenauer, H
影响因子：
2.3
作者：
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通讯作者：
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13.5
作者：
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6.2
作者：
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通讯作者：
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影响因子：
3.7
作者：
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通讯作者：
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