Cellular kinetics of hematopoietic cells with Sfpi1 deletion are present at different frequencies in bone-marrow and spleen in X-irradiated mice
Cellular kinetics of hematopoietic cells with Sfpi1 deletion are present at different frequencies in bone-marrow and spleen in X-irradiated mice
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具有 Sfpi1 缺失的造血细胞的细胞动力学在 X 射线照射的小鼠的骨髓和脾脏中以不同的频率存在
DOI：
10.1080/09553002.2020.1793018
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发表时间：
2020
影响因子：
2.6
通讯作者：
Kai Michiaki
中科院分区：
文献类型：
--
作者：
Etani Reo;Ojima Mitsuaki;Ariyoshi Kentaro;Fujishima Yohei;Kai Michiaki
研究目的：过去的几项研究表明，Sfpi 1基因（HDSG）的半合子缺失是放射诱导的急性髓细胞白血病（rAML）发展的起始事件。在这项研究中，我们研究的差异，在造血干细胞（HSC）富含造血细胞群体（HRCs）从骨髓（BM）和脾脏照射3戈伊X-raine.Materials和方法8周龄雄性C3 H小鼠照射全身X射线（1戈伊/min）3戈伊和小鼠在1，4，8，26周处死。结果与结论X线照射后1周，骨髓和脾脏中HDSG缺失的HRCs的频率均增加。照射后4 ~ 26周，骨髓中HDSG阳性HRCs的出现率逐渐下降，而脾脏中HDSG阳性HRCs的出现率在照射后26周仍保持较高水平。脾脏和股骨骨髓可能是rAML发生的主要部位。
PurposeSeveral past studies using a mouse model of radiation-induced AML (rAML) have shown that hemizygous deletion of theSfpi1gene (HDSG) is an initiating event for the development of rAML. In this study, we examined the difference in frequency ofHDSGin hematopoietic stem cells (HSCs) Rich hematopoietic Cell population (HRCs) from bone marrow (BM) and spleen of C3H mice irradiated with 3 Gy X-rays.Materials and methods8-weeks old male C3H mice were irradiated 3Gy of whole body X-ray (1 Gy/min) and mice were sacrificed at 1, 4, 8, and 26 weeks. Then, HSPCs were isolated from BM of femur and spleen, the frequency of HRCs withSfpi1gene deletion was analyzed by fluorescence in situ hybridization (FISH).Results and conclusionsThe frequency of HRCs withHDSGin both BM and spleen was increased 1 week after X-irradiation. Then, the frequency of HRCs withHDSGin BM showed a gradual decrease from 4 to 26 weeks, whereas HRCs withHDSGin spleen remained high, even at 26 weeks after X-irradiation.HDSGis less likely to be eliminated, particularly in the spleen, after X-irradiation. The spleen as well as BM of the femur may be major sites of rAML development.
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影响因子：
11.2
作者：
A. Upton;F. F. Wolff;J. Furth;A. Kimball
通讯作者：
A. Kimball
影响因子：
56.9
作者：
SCOTT, EW;SIMON, MC;SINGH, H
通讯作者：
SINGH, H
肾周脂肪肉瘤含有与肾细胞癌相关的髓外造血。
影响因子：
2.1
作者：
D. J. Lewis;J. Moul;S. C. Williams;I. Sesterhenn;E. Colón
通讯作者：
E. Colón
影响因子：
3.4
作者：
SEKI, M;YOSHIDA, K;NEMOTO, K
通讯作者：
NEMOTO, K
DOI：
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发表时间：
2004
期刊：
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影响因子：
--
作者：
通讯作者：
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