Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Qβ Conjugates as a Vaccine Candidate.
Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Qβ Conjugates as a Vaccine Candidate.
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DOI：
10.3390/ijms222212538
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发表时间：
2021-11-21
影响因子：
5.6
通讯作者：
Wang LX
中科院分区：
文献类型：
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作者：
Zong G;Toonstra C;Yang Q;Zhang R;Wang LX
The broadly neutralizing antibody PG9 recognizes a unique glycopeptide epitope in the V1V2 domain of HIV-1 gp120 envelope glycoprotein. The present study describes the design, synthesis, and antibody-binding analysis of HIV-1 V1V2 glycopeptide-Qβ conjugates as a mimic of the proposed neutralizing epitope of PG9. The glycopeptides were synthesized using a highly efficient chemoenzymatic method. The alkyne-tagged glycopeptides were then conjugated to the recombinant bacteriophage (Qβ), a virus-like nanoparticle, through a click reaction. Antibody-binding analysis indicated that the synthetic glycoconjugates showed significantly enhanced affinity for antibody PG9 compared with the monomeric glycopeptides. It was also shown that the affinity of the Qβ-conjugates for antibody PG9 was dependent on the density of the glycopeptide antigen display. The glycopeptide-Qβ conjugates synthesized represent a promising candidate of HIV-1 vaccine.
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影响因子：
3.5
作者：
Prabhu SK;Li C;Zong G;Zhang R;Wang LX
通讯作者：
Wang LX
影响因子：
4.9
作者：
Cai, Hui;Orwenyo, Jared;Wang, Lai-Xi
通讯作者：
Wang, Lai-Xi
DOI：
10.1126/science.1213256
发表时间：
2011-11-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Pejchal R;Doores KJ;Walker LM;Khayat R;Huang PS;Wang SK;Stanfield RL;Julien JP;Ramos A;Crispin M;Depetris R;Katpally U;Marozsan A;Cupo A;Maloveste S;Liu Y;McBride R;Ito Y;Sanders RW;Ogohara C;Paulson JC;Feizi T;Scanlan CN;Wong CH;Moore JP;Olson WC;Ward AB;Poignard P;Schief WR;Burton DR;Wilson IA
通讯作者：
Wilson IA
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
18.2
作者：
Cai H;Zhang R;Orwenyo J;Giddens J;Yang Q;LaBranche CC;Montefiori DC;Wang LX
通讯作者：
Wang LX