The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods.

The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods.
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DOI:
10.1371/journal.pone.0123690
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发表时间:
2015
期刊:
影响因子:
3.7
通讯作者:
Schouls LM
Schouls LM
中科院分区:
综合性期刊3区
文献类型:
--
作者:
van der Zwaluw K;de Haan A;Pluister GN;Bootsma HJ;de Neeling AJ;Schouls LM

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开发了一种新的表型测试,称为碳青霉烯灭活方法(CIM),用于在8小时内检测革兰氏阴性杆菌中的碳青霉烯酶活性。该方法与通过PCR检测编码碳青霉烯酶KPC、NDM、OXA-48、Vim、IMP和OXA-23的基因获得的结果高度一致。其允许可靠地检测由肠杆菌科物种中的各种基因编码的碳青霉烯酶活性(例如,肺炎克雷伯氏菌、大肠杆菌和阴沟肠杆菌),但也存在于非发酵菌铜绿假单胞菌和鲍曼不动杆菌中。CIM被证明是一种具有成本效益且高度稳健的表型筛选方法,可以可靠地检测碳青霉烯酶活性。
A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR to detect genes coding for the carbapenemases KPC, NDM, OXA-48, VIM, IMP and OXA-23. It allows reliable detection of carbapenemase activity encoded by various genes in species of Enterobacteriaceae (e.g., Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae), but also in non-fermenters Pseudomonas aeruginosa and Acinetobacter baumannii. The CIM was shown to be a cost-effective and highly robust phenotypic screening method that can reliably detect carbapenemase activity.
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