Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cells.
Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cells.
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沙门氏菌对培养上皮细胞的粘附和侵袭相关基因的克隆和分子表征。
DOI：
10.1111/j.1365-2958.1993.tb01100.x
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发表时间：
1993
影响因子：
3.6
通讯作者：
Galán,JE
中科院分区：
文献类型：
--
作者：
Altmeyer,RM;McNern,JK;Bossio,JC;Rosenshine,I;Finlay,BB;Galán,JE
我们的实验室已经独立地在霍乱沙门氏菌和鼠伤寒沙门氏菌中确定了一种基因，该基因是这些微生物有效黏附和进入培养的上皮细胞所必需的。将突变基因导入属于不同血清型的几个沙门氏菌菌株，使这些微生物缺乏对培养细胞的黏附和侵袭。这种作用在宿主适应血清型沙门氏菌、霍乱沙门氏菌和伤寒沙门氏菌中最为明显。该基因的核苷酸序列被我们命名为invH，编码一个含有一个信号序列的147个氨基酸的多肽。InvH预测多肽是高度保守的INS。鼠伤寒沙门氏菌霍乱，只有三个残留物。利用T7RNA聚合酶系统在大肠杆菌中表达了∼H基因，并观察到一条相对分子质量为16000的多肽，与预测的基因产物大小一致。纯化后，表达的多肽定位于细菌膜组分中。Southern和菌落杂交分析表明，除沙门氏菌外，所有受试沙门氏菌(分属37个血清型的91株)均存在invH基因，耶尔森氏菌、志贺氏菌、变形杆菌和几株侵袭性和致病性肠炎沙门氏菌均未检测到该基因的同源序列。科罗拉多。从这些下游。霍乱(但不是。检测到与插入序列IS3有广泛同源性的区域。
Our laboratories have independently identified a gene inSalmonella choleraesuisandSalmonella typhimuriumthat is necessary for efficient adherence and entry of these organisms into cultured epithelial cells. Introduction of a mutated gene into severalSalmonellastrains belonging to different serotypes rendered these organisms deficient for adherence and invasion of cultured cells. This effect was most pronounced in the host‐adapted serotypesSalmonella gallinarum, S. choleraesuis, andSalmonella typhi.The nucleotide sequence of this gene, which we have termedinvH, encodes a predicted 147‐amino‐acid polypeptide containing a signal sequence. The InvH predicted polypeptide is highly conserved inS. typhimuriumandS. choleraesuis, differing at only three residues. TheinvHgene was expressed inEscherichia coliusing a T7 RNA polymerase expression system and a polypeptide of ∼16000 molecular weight was observed, in agreement with the predicted size of its gene product. Upon fractionation, the expressed polypeptide was localized in the bacterial membrane fraction. Southern and colony hybridization analyses indicated that theinvHgene is present in allSalmonellastrains tested (91 strains belonging to 37 serotypes) with the exception of strains ofSalmonella arizonae.No homologous sequences were detected inYersinia, Shigella, Proteus, and several strains of enteroinvasive and enteropathogenicE. coli.Downstream from theS. choleraesuis(but notS. typhimurium) invHgene, a region with extensive homology to the insertion sequence IS3was detected.
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DNA 序列分析中使用核酸外切酶 III 进行单向消化。
影响因子：
--
作者：
S. Henikoff
通讯作者：
S. Henikoff
DOI：
10.1073/pnas.89.13.5976
发表时间：
1992-07-01
影响因子：
11.1
作者：
GINOCCHIO, C;PACE, J;GALAN, JE
通讯作者：
GALAN, JE
DOI：
10.1073/pnas.82.4.1074
发表时间：
1985-01-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
沙门氏菌产生的抗甘露糖血凝素的分布和特性。
影响因子：
3.8
作者：
Madelon C. Halula;Bruce A. D. Stocker
通讯作者：
Bruce A. D. Stocker
影响因子：
3.1
作者：
LIU, SL;EZAKI, T;YABUUCHI, E
通讯作者：
YABUUCHI, E