Purification and Characterization of Plantaricin YKX and Assessment of Its Inhibitory Activity Against Alicyclobacillus spp.
Purification and Characterization of Plantaricin YKX and Assessment of Its Inhibitory Activity Against Alicyclobacillus spp.
复制标题
Plantaricin YKX 的纯化、表征及其对脂环酸杆菌的抑制活性评估。
DOI：
10.3389/fmicb.2021.783266
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
Abd El-Aty AM
中科院分区：
文献类型：
--
作者：
Pei J;Jin W;Wang J;Huang Y;Li X;Zhang H;Zhang Y;Ramadan A;Abd El-Aty AM
消费者更喜欢食品标签上的天然而不是合成化学防腐剂。因此，确保这些天然防腐剂的安全性和有效性至关重要。耐热产孢脂环酸芽孢杆菌的出现。与果汁工业中的腐败问题有关。从汉中市传统酱菜中分离到一株细菌素产生菌YKX，经形态学、生理生化及基因型鉴定，该菌株为植物乳杆菌（Lactobacillusplantarum）。分离、纯化了一种稳定的细菌素植物菌素YKX，并测试了其对酸土脂环酸芽孢杆菌的效力。Plantaricin YKX是一种由14个氨基酸组成的多肽（Lys-Tyr-Gly-Asn-Gly-Leu-Ser-Arg-Ile-Phe-Ser-Ala-Leu-Lys）。其对供试细菌和真菌菌株的最小抑菌浓度（MIC）范围为16 - 64 μg/mL。它在pH 3-8时具有热稳定性和活性。流式细胞术数据和显微镜观察表明，植物菌素YKX可以增加细胞膜通透性，诱导钾离子渗漏和孔形成，并破坏细胞膜。它还影响了孢子萌发和愈创木酚的产生。acidoterrestris，可能是由于上调luxS基因与群体感应。
Consumers prefer natural over synthetic chemical preservatives on a food label. Therefore, it is crucial to ensure the safety and efficacy of such natural preservatives. The emergence of heat-resistant spore-forming Alicyclobacillus spp. has been associated with spoilage problems in the fruit juice industry. Herein, a bacteriocin-producing stain YKX was isolated from the traditional pickles in Hanzhong City, China, and it was identified as Lactobacillus plantarum by morphological, biochemical, physiological, and genotypic features. A stable bacteriocin, plantaricin YKX, was isolated, purified, and tested for its efficacy against Alicyclobacillus acidoterrestris. Plantaricin YKX is a 14-amino acid peptide (Lys-Tyr-Gly-Asn-Gly-Leu-Ser-Arg-Ile-Phe-Ser-Ala-Leu-Lys). Its minimal inhibitory concentrations (MICs) against the tested bacterial and fungal strains were ranged from 16 to 64 μg/mL. It is thermostable and active at pH 3–8. The flow cytometry data and microscopic observations suggested that plantaricin YKX can augment cell membrane permeability, induce potassium ion leakage and pore formation, and disrupt cell membranes. It also affects spore germination and guaiacol production of A. acidoterrestris, probably due to upregulation of the luxS gene linked to quorum sensing.
登录
查看更多内容
影响因子：
6
作者：
Evelyn;Silva, Filipa V. M.
通讯作者：
Silva, Filipa V. M.
影响因子：
6
作者：
An, Yu;Wang, Ying;Han, Xue
通讯作者：
Han, Xue
DOI：
10.1111/j.1365-2621.2006.01427.x
发表时间：
2008-02-01
影响因子：
3.3
作者：
Walker, Michelle;Phillips, Carol A.
通讯作者：
Phillips, Carol A.
影响因子：
6
作者：
Winkelstroeter, Lizziane Kretli;Tulini, Fabricio Luiz;De Martinis, Elaine C. P.
通讯作者：
De Martinis, Elaine C. P.
影响因子：
6.1
作者：
Hu, Meizhong;Zhao, Haizhen;Lu, Zhaoxin
通讯作者：
Lu, Zhaoxin