Identification and characterization of orthologs of AtNHX5 and AtNHX6 in Brassica napus.
Identification and characterization of orthologs of AtNHX5 and AtNHX6 in Brassica napus.
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DOI：
10.3389/fpls.2012.00208
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发表时间：
2012
影响因子：
5.6
通讯作者：
Gendall AR
中科院分区：
文献类型：
--
作者：
Ford BA;Ernest JR;Gendall AR
通过耐盐育种或引入耐盐性状来改良作物品种是盐碱地区提高作物产量的方法之一。通过对模式植物拟南芥的深入研究，人们已经获得了许多与耐盐性有关的基因的功能和重要性的大量信息。然而，A.在诸如甘蓝型油菜（油菜）的物种中的拟南芥直向同源物可能被证明是困难的，这是由于自从它们在大约2000万年前（MYA）分化以来已经发生了显著的基因组变化。最近公布的芜菁基因组为A.拟南芥和栽培芸苔属物种的同源物，并且有助于鉴定可能具有农学重要性的芸苔属物种直系同源物。钠氢反向转运蛋白（NHX）蛋白质转运钠或钾离子，以交换氢离子在另一个方向上穿过膜。以.在拟南芥中，NHX家族有八个成员，命名为AtNHX 1 -8，基于它们的亚细胞定位，可以将其细分为三个进化枝：质膜（PM）、细胞内I类（IC-I）和细胞内II类（IC-II）。在植物中，许多NHX蛋白是耐盐性的主要决定因素，并且通过将Na+运输出细胞质而起作用，否则Na+将在细胞质中积累到毒性水平。已经做了大量的工作来确定PM和IC-I进化枝成员在各种植物物种的耐盐性中的作用，但是对于IC-II进化枝的分析相对较少。这里我们描述B的识别。AtNHX 5和AtNHX 6的napus直系同源物，使用B. rapa基因组序列，宏观和微观同线性分析，比较表达和启动子基序分析，并强调这些多种方法的价值，在密切相关的物种与多个旁系同源物确定真正的直系同源物。
Improving crop species by breeding for salt tolerance or introducing salt tolerant traits is one method of increasing crop yields in saline affected areas. Extensive studies of the model plant species Arabidopsis thaliana has led to the availability of substantial information regarding the function and importance of many genes involved in salt tolerance. However, the identification and characterization of A. thaliana orthologs in species such as Brassica napus (oilseed rape) can prove difficult due to the significant genomic changes that have occurred since their divergence approximately 20 million years ago (MYA). The recently released Brassica rapa genome provides an excellent resource for comparative studies of A. thaliana and the cultivated Brassica species, and facilitates the identification of Brassica species orthologs which may be of agronomic importance. Sodium hydrogen antiporter (NHX) proteins transport a sodium or potassium ion in exchange for a hydrogen ion in the other direction across a membrane. In A. thaliana there are eight members of the NHX family, designated AtNHX1-8, that can be sub-divided into three clades, based on their subcellular localization: plasma membrane (PM), intracellular class I (IC-I) and intracellular class II (IC-II). In plants, many NHX proteins are primary determinants of salt tolerance and act by transporting Na+ out of the cytosol where it would otherwise accumulate to toxic levels. Significant work has been done to determine the role of both PM and IC-I clade members in salt tolerance in a variety of plant species, but relatively little analysis has been described for the IC-II clade. Here we describe the identification of B. napus orthologs of AtNHX5 and AtNHX6, using the B. rapa genome sequence, macro- and micro-synteny analysis, comparative expression and promoter motif analysis, and highlight the value of these multiple approaches for identifying true orthologs in closely related species with multiple paralogs.
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影响因子：
3.3
作者：
Bowers, K;Levi, BP;Stevens, TH
通讯作者：
Stevens, TH
影响因子：
5.6
作者：
Chanroj S;Wang G;Venema K;Zhang MW;Delwiche CF;Sze H
通讯作者：
Sze H
影响因子：
12.3
作者：
Mun JH;Kwon SJ;Yang TJ;Seol YJ;Jin M;Kim JA;Lim MH;Kim JS;Baek S;Choi BS;Yu HJ;Kim DS;Kim N;Lim KB;Lee SI;Hahn JH;Lim YP;Bancroft I;Park BS
通讯作者：
Park BS
影响因子：
11.6
作者：
Bassil, Elias;Ohto, Masa-aki;Blumwald, Eduardo
通讯作者：
Blumwald, Eduardo
DOI：
10.1073/pnas.2535513100
发表时间：
2003-12-23
影响因子：
11.1
作者：
Moore, RC;Purugganan, MD
通讯作者：
Purugganan, MD