Tal2c Activates the Expression of OsF3H(04g) to Promote Infection as a Redundant TALE of Tal2b in Xanthomonas oryzae pv. oryzicola.
Tal2c Activates the Expression of OsF3H(04g) to Promote Infection as a Redundant TALE of Tal2b in Xanthomonas oryzae pv. oryzicola.
复制标题
Tal2c 激活 OsF3H04g 的表达，作为 Tal2b 的冗余 TALE 在米黄单胞菌 pv 中促进感染。
DOI：
10.3390/ijms222413628
复制
发表时间：
2021-12-20
影响因子：
5.6
通讯作者：
Chu Z
中科院分区：
文献类型：
--
作者：
Wu T;Zhang H;Bi Y;Yu Y;Liu H;Yang H;Yuan B;Ding X;Chu Z
米黄单胞菌将转录激活因子样效应子 (TALE) 传递到植物细胞中以促进感染。遵循经济学原理，黄单胞菌中很少发现冗余的 TALE。此前，我们鉴定了 Tal2b，它可以激活水稻 2-氧化戊二酸依赖性双加氧酶基因 OsF3H03g 的表达，从而促进高毒力稻瘟病菌的感染。米兹科拉 HGA4。在这里，我们揭示了另一个簇状 TALE，Tal2c，也可作为靶向水稻 OsF3H04g（OsF3H03g 的同源物）的毒力因子。将 Tal2c 转移到 RS105 中诱导 OsF3H04g 的表达，这与水稻易感性的增加相一致。与野生型植物相比，过度表达 OsF3H04g 导致更高的敏感性和更少的水杨酸 (SA) 产量。此外，发现 CRISPR-Cas9 系统介导的 OsF3H03g 或 OsF3H04g 启动子中效应子结合元件的编辑可特异性增强对 Tal2b 或 Tal2c 转移菌株的抗性，但对 RS105 或 HGA4 的抗性没有影响。此外，转录组分析显示，与 OsF3H03g 过表达系中鉴定的基因相比，一些报道的 SA 相关和防御相关基因通常会改变 OsF3H04g 过表达系中的表达。总体而言，我们的结果揭示了 Xoc 致病毒力的功能冗余机制，其中串联 Tal2b 和 Tal2c 特异性靶向宿主基因的同源物，通过减少 SA 干扰水稻免疫。
Xanthomonas oryzae delivers transcription activator-like effectors (TALEs) into plant cells to facilitate infection. Following economic principles, the redundant TALEs are rarely identified in Xanthomonas. Previously, we identified the Tal2b, which activates the expression of the rice 2-oxoglutarate-dependent dioxygenase gene OsF3H03g to promote infection in the highly virulent strain of X. oryzae pv. oryzicola HGA4. Here, we reveal that another clustered TALE, Tal2c, also functioned as a virulence factor to target rice OsF3H04g, a homologue of OsF3H03g. Transferring Tal2c into RS105 induced expression of OsF3H04g to coincide with increased susceptibility in rice. Overexpressing OsF3H04g caused higher susceptibility and less salicylic acid (SA) production compared to wild-type plants. Moreover, CRISPR–Cas9 system-mediated editing of the effector-binding element in the promoters of OsF3H03g or OsF3H04g was found to specifically enhance resistance to Tal2b- or Tal2c-transferring strains, but had no effect on resistance to either RS105 or HGA4. Furthermore, transcriptome analysis revealed that several reported SA-related and defense-related genes commonly altered expression in OsF3H04g overexpression line compared with those identified in OsF3H03g overexpression line. Overall, our results reveal a functional redundancy mechanism of pathogenic virulence in Xoc in which tandem Tal2b and Tal2c specifically target homologues of host genes to interfere with rice immunity by reducing SA.
登录
查看更多内容
影响因子：
5.6
作者：
Farrow SC;Facchini PJ
通讯作者：
Facchini PJ
影响因子：
3.5
作者：
Li, Jinyun;Pang, Zhiqian;Wang, Nian
通讯作者：
Wang, Nian
影响因子：
11.6
作者：
Lu, Xuan;Zhang, Juan;Peters, Reuben J.
通讯作者：
Peters, Reuben J.
影响因子：
56.9
作者：
Boch, Jens;Scholze, Heidi;Bonas, Ulla
通讯作者：
Bonas, Ulla
影响因子：
5.2
作者：
Doucouré H;Pérez-Quintero AL;Reshetnyak G;Tekete C;Auguy F;Thomas E;Koebnik R;Szurek B;Koita O;Verdier V;Cunnac S
通讯作者：
Cunnac S