Single-gene lysis in the metagenomic era.
Single-gene lysis in the metagenomic era.
复制标题
宏基因组时代的单基因裂解。
DOI：
10.1016/j.mib.2020.09.015
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发表时间：
2020-08
影响因子：
5.4
通讯作者：
Young R
中科院分区：
文献类型：
--
作者：
Chamakura KR;Young R
小的裂解噬菌体（ssNA）使用单个蛋白（Sgl）引起宿主裂解。这3种表征良好的Sgls针对肽聚糖生物合成的不同步骤。近年来测序的ssNA基因组的过度扩增。有可能发现新的蛋白质抗生素。小的裂解噬菌体（微病毒科和利维病毒科）用单个基因的产物进行细菌裂解。三种被充分研究的单基因裂解（Sgl）蛋白（φX174的E， Qβ的a2和噬菌体M的Lys M）缺乏直接的水解活性，并且已被证明作为“蛋白质抗生素”的功能，分别作为保守肽聚糖（PG）生物合成酶MurA， MraY和MurJ的非竞争性抑制剂。第四个是MS2的蛋白L，它不抑制PG的生物合成，而是通过一种未知的机制引发宿主自溶反应。元组学方法的最新进展导致了小型裂解噬菌体可用基因组的爆炸式增长。在数千个新基因组中，只有一个注释的Sgl与已知的Sgl （MS2的L）具有一定的序列相似性，突出了Sgls的多样性。新的可用的基因组空间是发现新的Sgls的未开发资源。
HighlightsSmall lytic phages (ssNA) use a single protein (Sgl) to cause host lysis.The 3 well-characterized Sgls target different steps in peptidoglycan biosynthesis.Hyperexpansion of the sequenced ssNA genomes in the recent years.Potential to discover new protein antibiotics.The small lytic phages (Microviridae and Leviviridae), effect bacterial lysis with the product of a single gene. The three well-studied single-gene lysis (Sgl) proteins (E of φX174, A 2 of Qβ, and Lys M of phage M) lack direct muralytic activity, and have been shown to function as ‘protein antibiotics’ by acting as noncompetitive inhibitors of conserved peptidoglycan (PG) biosynthesis enzymes, MurA, MraY, and MurJ respectively. The fourth, protein L of MS2, does not inhibit PG biosynthesis but instead is hypothesized to trigger host autolytic response through an unknown mechanism. Recent advances in meta-omics approaches have led to an explosion in the available genomes of small lytic phages. Of the thousands of new genomes, only one annotated Sgl shared some sequence similarity with a known Sgl (L of MS2), highlighting the diversity in Sgls. The newly available genomic space serves as an untapped resource for discovering novel Sgls.
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DOI：
10.1073/pnas.1707102114
发表时间：
2017-10-01
影响因子：
11.1
作者：
Cui, Zhicheng;Gorzelnik, Karl V.;Zhang, Junjie
通讯作者：
Zhang, Junjie
影响因子：
3.2
作者：
Kannoly, Sherin;Shao, Yongping;Wang, Ing-Nang
通讯作者：
Wang, Ing-Nang
影响因子：
4.4
作者：
Kim, Min-Soo;Park, Eun-Jin;Bae, Jin-Woo
通讯作者：
Bae, Jin-Woo
影响因子：
5.4
作者：
Kazaks, Andris;Voronkova, Tatyana;Tars, Kaspars
通讯作者：
Tars, Kaspars
影响因子：
3.8
作者：
BRADLEY, DE;DEWAR, CA;ROBERTSON, D
通讯作者：
ROBERTSON, D