Increased maternal nutrition increases leptin expression in perirenal and subcutaneous adipose tissue in the postnatal lamb.
Increased maternal nutrition increases leptin expression in perirenal and subcutaneous adipose tissue in the postnatal lamb.
复制标题
母体营养的增加会增加产后羔羊肾周和皮下脂肪组织中瘦素的表达。
作者：
Beverly S. Muhlhausler;J. Duffield;I. C. Mcmillen
本研究验证了这一假设，即出生前母体营养水平的增加会导致出生后早期脂肪组织中脂肪形成、脂肪形成和脂肪因子基因表达的改变。妊娠母羊在妊娠后期以维持能量需求或高于维持能量需求约50%的水平喂养，并在出生后第30天测定后代肾周（PAT）和皮下脂肪组织（SCAT）中过氧化物酶体增殖物激活受体（PPAR）-γ、脂蛋白脂酶（LPL）、甘油-3-磷酸脱氢酶（G3PDH）、脂联素和瘦素mRNA的表达。在喂养良好的母羊的羔羊中，相对SCAT质量较高（40.0 +/-4.0 vs. 22.8 +/-3.3 g/kg，P <0.05），并且与出生后前24小时的血浆胰岛素以及G3 PDH和LPL表达直接相关。在SCAT和PAT贮库中，Leptin mRNA的表达量均显著高于对照组（P <0.05）。PPARgamma脂联素、LPL和G3PDH mRNA表达在两个贮库中的良好喂养组和对照组之间没有差异。SCAT中PPARgamma的相对表达与出生后24h内血浆胰岛素浓度呈正相关（r（2）= 0.23; P <0.05），G3PDH和LPL表达与PPARgamma表达也呈正相关（r（2）= 0.27; P <0.05）。我们已经证明，暴露于增加产前营养增加瘦素表达在1个月大的PAT和SCAT。这项研究的结果提供的证据表明，出生前和出生后立即营养环境可以影响出生后早期脂肪组织的发育。
The present study tested the hypothesis that exposure to an increased level of maternal nutrition before birth results in altered expression of adipogenic, lipogenic, and adipokine genes in adipose tissue in early postnatal life. Pregnant ewes were fed either at or approximately 50% above maintenance energy requirements during late pregnancy, and quantitative RT-PCR was used to measure peroxisome proliferator-activated receptor (PPAR)-gamma, lipoprotein lipase (LPL), glycerol-3-phosphate-dehydrogenase (G3PDH), adiponectin, and leptin mRNA expression in perirenal (PAT) and sc adipose tissue (SCAT) in the offspring on postnatal d 30. Relative SCAT mass was higher in lambs of well-fed ewes (40.0 +/- 4.0 vs. 22.8 +/- 3.3 g/kg, P < 0.05) and was directly related to plasma insulin in the first 24 h after birth and to G3PDH and LPL expression. The expression of leptin mRNA in both the SCAT and PAT depots was higher (P < 0.05) in lambs of well-fed ewes. PPARgamma adiponectin, LPL, and G3PDH mRNA expression were not, however, different between well-fed and control groups in either depot. Relative PPARgamma expression in SCAT was directly related to plasma insulin concentrations in the first 24 h after birth (r(2) = 0.23; P < 0.05), and G3PDH and LPL expressions were also positively correlated with PPARgamma expression (r(2) = 0.27; P < 0.05). We have demonstrated that exposure to increased prenatal nutrition increases leptin expression at 1 month of age in both PAT and SCAT. The results of this study provide evidence that the nutritional environment before and immediately after birth can influence the development of adipose tissue in early postnatal life.
DOI：
10.1016/s0002-9378(03)00828-7
发表时间：
2003-12-01
影响因子：
9.8
作者：
Catalano, PM;Thomas, A;Amini, SB
通讯作者：
Amini, SB
影响因子：
4.8
作者：
A. Chawla;E. Schwarz;D. Dimaculangan;M. Lazar
通讯作者：
A. Chawla;E. Schwarz;D. Dimaculangan;M. Lazar