Multifunctional iron platinum stealth immunomicelles: targeted detection of human prostate cancer cells using both fluorescence and magnetic resonance imaging.
Multifunctional iron platinum stealth immunomicelles: targeted detection of human prostate cancer cells using both fluorescence and magnetic resonance imaging.
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DOI：
10.1007/s11051-011-0439-3
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发表时间：
2011-10-01
影响因子：
2.5
通讯作者：
Sillerud, Laurel O.
中科院分区：
文献类型：
--
作者：
Taylor, Robert M.;Huber, Dale L.;Monson, Todd C.;Ali, Abdul-Mehdi S.;Bisoffi, Marco;Sillerud, Laurel O.
关键词：
超顺磁性氧化铁纳米颗粒（SPION）是造影剂增强磁共振成像（MRI）中最常见的造影剂类型。尽管如此，仍有很大的改进空间，并且需要具有增加的MRI弛豫率的纳米颗粒来增加应用于包括癌症在内的各种医疗状况的MRI中的对比度增强。我们报告了超顺磁性铁铂纳米颗粒（SIPPs）的合成和随后的封装使用聚乙二醇化磷脂，以创建隐形免疫胶束（DSPE-SIPPs），可以特异性地针对人类前列腺癌细胞系，并使用MRI和荧光成像检测。SIPP核和DSPE-SIPPs的直径分别为8.5 ± 1.6 nm和42.9 ± 8.2 nm，SIPPs的磁矩为120 A m2/kg铁。将抗前列腺特异性膜抗原（PSMA）的单克隆抗体J591与DSPE-SIPPs偶联（J591-DSPE-SIPPs），并使用荧光共聚焦显微镜证明J591-DSPE-SIPPs对表达PSMA的人前列腺癌细胞系的特异性靶向。在4.7特斯拉下测量的DSPE-SIPPs的横向弛豫率为300.6 ± 8.5 s−1 mM−1，比市售SPION（23.8 ± 6.9 s−1 mM−1）好13倍，比Feridex®和Resovist®的弛豫率好约3倍。我们的数据表明，J591-DSPE-SIPP在体外特异性靶向人类前列腺癌细胞，是T2加权MRI中的上级造影剂，并且可以使用荧光成像进行检测。据我们所知，这是第一个报告的多功能SIPP胶束的合成和使用SIPP的前列腺癌的特异性检测。
Superparamagnetic iron oxide nanoparticles (SPIONs) are the most common type of contrast agents used in contrast agent-enhanced magnetic resonance imaging (MRI). Still, there is a great deal of room for improvement, and nanoparticles with increased MRI relaxivities are needed to increase the contrast enhancement in MRI applied to various medical conditions including cancer. We report the synthesis of superparamagnetic iron platinum nanoparticles (SIPPs) and subsequent encapsulation using PEGylated phospholipids to create stealth immunomicelles (DSPE-SIPPs) that can be specifically targeted to human prostate cancer cell lines and detected using both MRI and fluorescence imaging. SIPP cores and DSPE-SIPPs were 8.5 ± 1.6 nm and 42.9 ± 8.2 nm in diameter, respectively, and the SIPPs had a magnetic moment of 120 A m2/kg iron. J591, a monoclonal antibody against prostate specific membrane antigen (PSMA), was conjugated to the DSPE-SIPPs (J591-DSPE-SIPPs), and specific targeting of J591-DSPE-SIPPs to PSMA-expressing human prostate cancer cell lines was demonstrated using fluorescence confocal microscopy. The transverse relaxivity of the DSPE-SIPPs, measured at 4.7 Tesla, was 300.6 ± 8.5 s−1 mM−1, which is 13-fold better than commercially available SPIONs (23.8 ± 6.9 s−1 mM−1) and ~3-fold better than reported relaxivities for Feridex® and Resovist®. Our data suggest that J591-DSPE-SIPPs specifically target human prostate cancer cells in vitro, are superior contrast agents in T2-weighted MRI, and can be detected using fluorescence imaging. To our knowledge, this is the first report on the synthesis of multifunctional SIPP micelles and using SIPPs for the specific detection of prostate cancer.
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影响因子：
3.2
作者：
INOMATA, K;SAWA, T;HASHIMOTO, S
通讯作者：
HASHIMOTO, S
影响因子：
82.9
作者：
Lee, Jae-Hyun;Huh, Yong-Min;Cheon, Jinwoo
通讯作者：
Cheon, Jinwoo
影响因子：
--
作者：
Puerto Morales, Maria;Bedard, Matthieu F.;Parak, Wolfgang J.
通讯作者：
Parak, Wolfgang J.
影响因子：
--
作者：
Bhalerao, G. M.;Sinha, A. K.;Srivastava, A. K.
通讯作者：
Srivastava, A. K.
DOI：
10.1002/cyto.990110203
发表时间：
1990-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
MILTENYI, S;MULLER, W;RADBRUCH, A
通讯作者：
RADBRUCH, A