Comparative proteomic analysis of cucumber powdery mildew resistance between a single-segment substitution line and its recurrent parent
Comparative proteomic analysis of cucumber powdery mildew resistance between a single-segment substitution line and its recurrent parent
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单段替代系与其轮回亲本之间黄瓜白粉病抗性的比较蛋白质组学分析
DOI：
10.1038/s41438-019-0198-3
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发表时间：
2019-10
影响因子：
8.7
通讯作者：
Chen Xuehao
中科院分区：
文献类型：
--
作者：
Xu Xuewen;Liu Xueli;Yan Yali;Wang Wei;Gebretsadik Kiros;Qi Xiaohua;Xu Qiang;Chen Xuehao
白粉病被认为是造成世界范围内黄瓜产量损失和品质下降的主要原因，但对白粉病抗性的分子基础仍知之甚少。以感病黄瓜自交系D8为遗传背景，育成了一个具有显性抗白粉病基因的片段代换系SSL508-28。替换片段包含860个基因。使用基于iTRAQ的比较蛋白质组学技术对接种PM和未处理(对照)的D8和SSL508-28的蛋白质组进行了定位。SSL508-28的差异调节蛋白(DRPs)数几乎是D8的3倍。14个DRP位于替换节段间隔区。比较基因表达分析表明，根瘤素相关蛋白1(Nrp1)可能是一个很好的PM抗性候选基因。基因本体论分析表明，在抗病品系SSL508-28中，参与四吡咯生物合成过程、硫代谢过程和细胞氧化还原动态平衡的DRPs具有特异的富集性。KEGG术语中的DRPs在PM侵染时两个品系的光合作用都增加了，这表明黄瓜在最初阶段对PM攻击的反应策略可能不同于其他作物。过氧化氢和超氧阴离子产生量和净光合速率的测定与蛋白质丰度的变化一致，表明蛋白质组学结果是可靠的。在相同的实验设计下，iTRAQ测得的DRPs与RNA-seq测得的相应基因表达变化之间的相关性较差。综上所述，这些发现提高了对黄瓜对PM侵染反应的分子机制的理解。
Powdery mildew (PM) is considered a major cause of yield losses and reduced quality in cucumber worldwide, but the molecular basis of PM resistance remains poorly understood. A segment substitution line, namely, SSL508-28, was developed with dominant PM resistance in the genetic background of PM-susceptible cucumber inbred line D8. The substituted segment contains 860 genes. An iTRAQ-based comparative proteomic technology was used to map the proteomes of PM-inoculated and untreated (control) D8 and SSL508-28. The number of differentially regulated proteins (DRPs) in SSL508-28 was almost three times higher than that in D8. Fourteen DRPs were located in the substituted segment interval. Comparative gene expression analysis revealed that nodulin-related protein 1 (NRP1) may be a good candidate for PM resistance. Gene Ontology enrichment analysis showed that DRPs functioning in tetrapyrrole biosynthetic process, sulfur metabolic process and cell redox homeostasis were specifically enriched in the resistant line SSL508-28. DRPs categorized in the KEGG term photosynthesis increased in both lines upon PM infection, suggesting that the strategies used by cucumber may be different from those used by other crops to react to PM attacks at the initial stage. The measurement of hydrogen peroxide and superoxide anion production and net photosynthetic rate were consistent with the changes in protein abundance, suggesting that the proteomic results were reliable. There was a poor correlation between DRPs measured by iTRAQ and the corresponding gene expression changes measured by RNA-seq with the same experimental design. Taken together, these findings improve the understanding of the molecular mechanisms underlying the response of cucumber to PM infection.
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影响因子：
3.8
作者：
Fujimori, Nozomi;Suzuki, Nana;Suzuki, Shunji
通讯作者：
Suzuki, Shunji
CsMLO1 的功能缺失突变赋予黄瓜 (Cucumis sativus L.) 持久的白粉病抗性
影响因子：
5.6
作者：
Nie J;Wang Y;He H;Guo C;Zhu W;Pan J;Li D;Lian H;Pan J;Cai R
通讯作者：
Cai R
影响因子：
5.3
作者：
Gong Q;Yang Z;Wang X;Butt HI;Chen E;He S;Zhang C;Zhang X;Li F
通讯作者：
Li F
影响因子：
7.2
作者：
Rao, MV;Davis, KR
通讯作者：
Davis, KR
影响因子：
9.5
作者：
Jos H. M. Schippers;C. Foyer;Joost T. van Dongen
通讯作者：
Jos H. M. Schippers;C. Foyer;Joost T. van Dongen