Modulation of bone metabolism by two chemically distinct lipopolysaccharide fractions from Bacteroides gingivalis
Modulation of bone metabolism by two chemically distinct lipopolysaccharide fractions from Bacteroides gingivalis
复制标题
牙龈拟杆菌中两种化学性质不同的脂多糖组分对骨代谢的调节
作者：
S. Millar;E. Goldstein;M. Levine;E. Hausmann
从牙龈拟杆菌381中分离出两种脂多糖（LPS）。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳不仅证明了每个物种的异质性，而且还表明它们代表了高分子量和低分子量的LPS实体。虽然两者都含有相同的碳水化合物和脂肪酸成分，但这些成分的比例在LPS物种之间有所不同。这两个物种在调节骨吸收和骨胶原蛋白和非胶原蛋白合成方面的直接作用已被研究。在骨吸收实验中，这两个物种刺激了45Ca从预标记的胎鼠骨骼中释放，浓度范围为0.5至3.0微克/毫升。两种脂多糖在10微克/毫升的浓度下也能使胶原蛋白的形成减少30 - 40%。明尼苏达沙门氏菌LPS浓度为10微克/毫升时，也有相同量级的反应。高分子量的LPS物种也显著抑制非胶原蛋白的形成。这是首次报道来自牙龈B.龈381（一种可疑的牙周病原体）的LPS抑制骨胶原形成，并与骨吸收能力一起进一步暗示LPS与牙周病相关的牙槽骨丢失。
Two separate species of lipopolysaccharide (LPS) from Bacteroides gingivalis 381 have been isolated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated not only the heterogeneity of each species, but also that they represented high- and low-molecular-weight LPS entities. Although both contained the same carbohydrate and fatty acid components, the proportions of these differed between the LPS species. The direct effects of these two species in modulation of bone resorption and bone collagen and noncollagen protein synthesis have been examined. In a bone resorption assay, these two species stimulated 45Ca release from prelabeled fetal rat bones in a concentration range of 0.5 to 3.0 micrograms/ml. The two LPS species also elicited a 30 to 40% reduction in collagen protein formation at 10 micrograms/ml. Responses of the same order of magnitude were observed with LPS from Salmonella minnesota at 10 micrograms/ml. The higher-molecular-weight LPS species also significantly inhibited noncollagen protein formation. This is the first report that LPS from B. gingivalis 381, a suspected periodontal pathogen, inhibits bone collagen formation and, in conjunction with the bone resorption potency, further implicates LPS in alveolar bone loss associated with periodontal disease.
人腮腺唾液中富含脯氨酸的糖蛋白的碳水化合物链结构。
DOI：
10.1016/0006-291x(82)91331-6
发表时间：
1982
影响因子：
3.1
作者：
Reddy,MS;Levine,MJ;Tabak,LA
通讯作者：
Tabak,LA
影响因子：
3.5
作者：
NAIR, BC;MAYBERRY, WR;HAUSMANN, E
通讯作者：
HAUSMANN, E
喉切除者气管支气管粘蛋白的分离和表征。
影响因子：
3.1
作者：
Tabak,LA;Reddy,MS;Monte,LD;Levine,MJ
通讯作者：
Levine,MJ