Aberrant N-Glycosylation Profile of Serum Immunoglobulins is a Diagnostic Biomarker of Urothelial Carcinomas.
Aberrant N-Glycosylation Profile of Serum Immunoglobulins is a Diagnostic Biomarker of Urothelial Carcinomas.
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DOI：
10.3390/ijms18122632
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发表时间：
2017-12-06
影响因子：
5.6
通讯作者：
Ohyama C
中科院分区：
文献类型：
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作者：
Tanaka T;Yoneyama T;Noro D;Imanishi K;Kojima Y;Hatakeyama S;Tobisawa Y;Mori K;Yamamoto H;Imai A;Yoneyama T;Hashimoto Y;Koie T;Tanaka M;Nishimura SI;Kurauchi S;Takahashi I;Ohyama C
本研究的目的是确定异常N-糖基化血清免疫球蛋白（Ig）是否可以作为尿路上皮癌（UC）的诊断标志物。在2009年至2016年期间，我们从我们的血清库中随机获得了237名UC和96名前列腺癌患者的血清作为其他癌症对照，并从339名健康志愿者（HV）中获得了Iwaki健康促进项目社区居民志愿者的对照。通过高通量N-糖组学测定Ig上总共32种类型的N-聚糖水平，并通过多变量判别分析进行分析。我们发现了五种UC相关的异常N-聚糖在Ig上的变化，并且还发现在UC患者中Ig上的无唾液酸二等分GlcNAc型N-聚糖显著积累。通过组合Ig上的五种N-聚糖建立的诊断性N-聚糖评分（dNGScore）以92.8%的灵敏度和97.2%的特异性区分UC患者与HV和前列腺癌（PC）患者。UC检测的dNGScore曲线下面积（AUC）为0.969，远上级尿细胞学（AUC，0.707）和血尿（AUC，0.892）。此外，dNGScore可以检测血尿和尿细胞学阴性患者。发现基于Ig的异常N-糖基化特征的dNG评分是UC的有希望的诊断生物标志物。
The aim of this study to determine whether the aberrant N-glycosylated serum immunoglobulins (Igs) can be applied as a diagnostic marker of urothelial carcinoma (UC). Between 2009 and 2016, we randomly obtained serum available from 237 UC and also 96 prostate cancer as other cancer controls from our serum bank and also obtained—from 339 healthy volunteers (HV)—controls obtained from community-dwelling volunteers in Iwaki Health Promotion Project. A total of 32 types of N-glycan levels on Igs were determined by high-throughput N-glycomics and analyzed by multivariable discriminant analysis. We found five UC-associated aberrant N-glycans changes on Igs and also found that asialo-bisecting GlcNAc type N-glycan on Igs were significantly accumulated in UC patients. The diagnostic N-glycan Score (dNGScore) established by combination of five N-glycans on Igs discriminated UC patients from HV and prostate cancer (PC) patients with 92.8% sensitivity and 97.2% specificity. The area under the curve (AUC) for of the dNGScore was 0.969 for UC detection that was much superior to that of urine cytology (AUC, 0.707) and hematuria (AUC, 0.892). Furthermore, dNGScore can detect hematuria and urine cytology negative patients. The dNGscore based on aberrant N-glycosylation signatures of Igs were found to be promising diagnostic biomarkers of UCs.
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影响因子：
--
作者：
Hatakeyama S;Amano M;Tobisawa Y;Yoneyama T;Tsushima M;Hirose K;Yoneyama T;Hashimoto Y;Koie T;Saitoh H;Yamaya K;Funyu T;Nishimura S;Ohyama C
通讯作者：
Ohyama C
影响因子：
4
作者：
Narita T;Hatakeyama S;Yoneyama T;Narita S;Yamashita S;Mitsuzuka K;Sakurai T;Kawamura S;Tochigi T;Takahashi I;Nakaji S;Tobisawa Y;Yamamoto H;Koie T;Tsuchiya N;Habuchi T;Arai Y;Ohyama C
通讯作者：
Ohyama C
影响因子：
4
作者：
Kazuno S;Furukawa J;Shinohara Y;Murayama K;Fujime M;Ueno T;Fujimura T
通讯作者：
Fujimura T
DOI：
10.1007/bf01857227
发表时间：
1988-01-01
期刊：
SPRINGER SEMINARS IN IMMUNOPATHOLOGY
影响因子：
--
作者：
RADEMACHER, TW;PAREKH, RB;ROITT, I
通讯作者：
ROITT, I
影响因子：
--
作者：
Kirwan A;Utratna M;O'Dwyer ME;Joshi L;Kilcoyne M
通讯作者：
Kilcoyne M