Composite mobile genetic elements disseminating macrolide resistance in Streptococcus pneumoniae.
Composite mobile genetic elements disseminating macrolide resistance in Streptococcus pneumoniae.
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DOI：
10.3389/fmicb.2015.00026
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发表时间：
2015
影响因子：
5.2
通讯作者：
Stephens DS
中科院分区：
文献类型：
--
作者：
Chancey ST;Agrawal S;Schroeder MR;Farley MM;Tettelin H;Stephens DS
肺炎链球菌对大环内酯类抗生素耐药始于上世纪90年代初的S，由erm(B)编码的核糖核酸甲基酶和大环内酯外排基因mef(E)和me1被鉴定为耐药的决定因素。这些基因在移动的肺炎球菌中传播，通常是由多个较小的分子组成的嵌合分子。为了更好地了解编码大环内酯类耐药性的各种元素以及它们在结合疫苗前和结合后时代是如何演变的，我们分析了1994年至2011年亚特兰大121株侵袭性和10株携带性分离株的基因组，以寻找参与大环内酯类耐药性传播的移动元件。选择这些分离株是为了提供对抗生素耐药肺炎球菌遗传变异性的广泛覆盖，其中包括100株对大环内酯类耐药的侵袭性分离株。在大环内酯类基因组装(Mega)上携带MEF(E)和MEL的Tn916样元件和在Erm(B)元件上携带Erm(B)的Tn916样元件和Tn917被整合到肺炎球菌染色体骨架和更大的Tn5253类复合元件中。本文所报道的结果包括鉴定新的MegA插入位点和鉴定肺炎球菌染色体和更大的复合元件中的Tn916类似元件的插入位点。数据表明，与重组相比，通过共轭整合元素的情况并不多见。因此，结合移动元件似乎允许肺炎球菌从远亲细菌获得DNA，但一旦整合到肺炎球菌基因组中，转化和重组是新DNA在肺炎球菌种群中传播的主要机制。
Macrolide resistance in Streptococcus pneumoniae emerged in the U.S. and globally during the early 1990's. The RNA methylase encoded by erm(B) and the macrolide efflux genes mef(E) and mel were identified as the resistance determining factors. These genes are disseminated in the pneumococcus on mobile, often chimeric elements consisting of multiple smaller elements. To better understand the variety of elements encoding macrolide resistance and how they have evolved in the pre- and post-conjugate vaccine eras, the genomes of 121 invasive and ten carriage isolates from Atlanta from 1994 to 2011 were analyzed for mobile elements involved in the dissemination of macrolide resistance. The isolates were selected to provide broad coverage of the genetic variability of antibiotic resistant pneumococci and included 100 invasive isolates resistant to macrolides. Tn916-like elements carrying mef(E) and mel on the Macrolide Genetic Assembly (Mega) and erm(B) on the erm(B) element and Tn917 were integrated into the pneumococcal chromosome backbone and into larger Tn5253-like composite elements. The results reported here include identification of novel insertion sites for Mega and characterization of the insertion sites of Tn916-like elements in the pneumococcal chromosome and in larger composite elements. The data indicate that integration of elements by conjugation was infrequent compared to recombination. Thus, it appears that conjugative mobile elements allow the pneumococcus to acquire DNA from distantly related bacteria, but once integrated into a pneumococcal genome, transformation and recombination is the primary mechanism for transmission of novel DNA throughout the pneumococcal population.
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影响因子：
5.4
作者：
Croucher NJ;Hanage WP;Harris SR;McGee L;van der Linden M;de Lencastre H;Sá-Leão R;Song JH;Ko KS;Beall B;Klugman KP;Parkhill J;Tomasz A;Kristinsson KG;Bentley SD
通讯作者：
Bentley SD
影响因子：
3.6
作者：
Brown, JS;Gilliland, SM;Holden, DW
通讯作者：
Holden, DW
影响因子：
11.8
作者：
Klugman KP;Lonks JR
通讯作者：
Lonks JR
DOI：
10.1099/00221287-144-11-3049
发表时间：
1998-11-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Enright, MC;Spratt, BG
通讯作者：
Spratt, BG
影响因子：
4.9
作者：
Chancey, Scott T.;Zhou, Xiaoliu;Stephens, David S.
通讯作者：
Stephens, David S.