Multilayer pixel super-resolution lensless in-line holographic microscope with random sample movement.
Multilayer pixel super-resolution lensless in-line holographic microscope with random sample movement.
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DOI：
10.1038/s41598-017-13134-4
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发表时间：
2017-10-06
影响因子：
4.6
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Wang M;Feng S;Wu J
我们报告了一种多层无透镜在线全息显微镜（LIHM），通过使用像素超分辨率技术和随机样本移动来提高成像分辨率。在我们的成像系统中，激光束照射样品，位于样品后面的 CMOS 成像传感器记录在线全息图以进行图像重建。在成像过程中，用手随机移动样品并顺序获取在线全息图。然后，通过应用像素超分辨率（PSR）技术，从多个低分辨率直列全息图获得的增强分辨率全息图重建样本图像。我们以美国空军（USAF）目标为样本，通过数值模拟和实验研究了分辨率增强效果。我们还表明，通过对中间层的丝状藻和顶层和底层的直径为2μm的微球制成的三层样品进行成像，可以获得多层像素超分辨率图像。我们的像素超分辨率 LIHM 为显微成像提供了紧凑且低成本的解决方案，并且在许多生物医学应用中具有广阔的前景。
We report a multilayer lensless in-line holographic microscope (LIHM) with improved imaging resolution by using the pixel super-resolution technique and random sample movement. In our imaging system, a laser beam illuminated the sample and a CMOS imaging sensor located behind the sample recorded the in-line hologram for image reconstruction. During the imaging process, the sample was moved by hand randomly and the in-line holograms were acquired sequentially. Then the sample image was reconstructed from an enhanced-resolution hologram obtained from multiple low-resolution in-line holograms by applying the pixel super-resolution (PSR) technique. We studied the resolution enhancement effects by using the U.S. Air Force (USAF) target as the sample in numerical simulation and experiment. We also showed that multilayer pixel super-resolution images can be obtained by imaging a triple-layer sample made with the filamentous algae on the middle layer and microspheres with diameter of 2 μm on the top and bottom layers. Our pixel super-resolution LIHM provides a compact and low-cost solution for microscopic imaging and is promising for many biomedical applications.
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影响因子：
6.1
作者：
Seo S;Su TW;Tseng DK;Erlinger A;Ozcan A
通讯作者：
Ozcan A
影响因子：
6.1
作者：
Bishara W;Sikora U;Mudanyali O;Su TW;Yaglidere O;Luckhart S;Ozcan A
通讯作者：
Ozcan A
影响因子：
6.1
作者：
Lee SA;Yang C
通讯作者：
Yang C
影响因子：
1.3
作者：
Yu, Xiao;Hong, Jisoo;Kim, Myung K.
通讯作者：
Kim, Myung K.
DOI：
10.1038/lsa.2016.60
发表时间：
2016-04
期刊：
Light, science & applications
影响因子：
--
作者：
Luo W;Zhang Y;Feizi A;Göröcs Z;Ozcan A
通讯作者：
Ozcan A