Development of dorsal-ventral polarity in the optic vesicle and its presumptive role in eye morphogenesis as shown by embryonic transplantation and in ovo explant culturing.
Development of dorsal-ventral polarity in the optic vesicle and its presumptive role in eye morphogenesis as shown by embryonic transplantation and in ovo explant culturing.
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视泡背腹极性的发育及其在眼睛形态发生中的推定作用，如胚胎移植和卵外植体培养所示。
作者：
T. Uemonsa;K. Sakagami;K. Yasuda;M. Araki
早期视泡的背侧和腹侧特化似乎在眼睛的正常发育中起着至关重要的作用。在本研究中，我们进行了胚胎移植和器官培养的鸡视泡，以探讨背腹（D-V）极性是如何建立在视泡和什么样的作用，这种极性在适当的眼睛发育。切割左视泡并倒置移植到宿主鸡胚的右眼腔内。这种方法确保D-V极性逆转，同时前后轴保持正常。结果表明，随着移植胚胎期从6体节到18体节，脉络膜裂的位置由正常（腹侧）变为异位。与此同时，视泡的形状和Pax 2和Tbx 5的表达模式，视泡的腹侧和背侧区域的标记基因，分别伴随着以类似的方式改变。关键时期是8- 14体节阶段之间，在此期间，极性似乎是逐渐确定的。卵内视泡培养表明，视泡的D-V极性和脉络膜裂的形成已经由10体节期所确定。这些结果表明，视泡的D-V极性是在前脑中线部分信号的影响下，在8- 14体节阶段逐渐建立起来的。推测的信号似乎是从视泡内的近端区域传输到远端区域。在10体节期前后移植的视泡发育中观察到一个严重的异常：视杯形成受到干扰，随后神经视网膜和色素上皮不能正常发育。我们的结论是D-V极性的建立在视泡中起着至关重要的作用，神经视网膜和色素上皮的图案和分化。
Dorsal and ventral specification in the early optic vesicle appears to play a crucial role in the proper development of the eye. In the present study, we performed embryonic transplantation and organ culturing of the chick optic vesicle in order to investigate how the dorsal-ventral (D-V) polarity is established in the optic vesicle and what role this polarity plays in proper eye development. The left optic vesicle was cut and transplanted inversely in the right eye cavity of host chick embryos. This method ensured that the D-V polarity was reversed while the anteroposterior axis remained normal. The results showed that the location of the choroid fissure was altered from the normal (ventral) to ectopic positions as the embryonic stage of transplantation progressed from 6 to 18 somites. At the same time, the shape of the optic vesicle and the expression patterns of Pax2 and Tbx5, marker genes for ventral and dorsal regions of the optic vesicle, respectively, changed concomitantly in a similar way. The crucial period was between the 8- and 14-somite stages, and during this period the polarity seemed to be gradually determined. In ovo explant culturing of the optic vesicle showed that the D-V polarity and choroid fissure formation were already specified by the 10-somite stage. These results indicate that the D-V polarity of the optic vesicle is established gradually between 8- and 14-somite stages under the influence of signals derived from the midline portion of the forebrain. The presumptive signal(s) appeared to be transmitted from proximal to distal regions within the optic vesicle. A severe anomaly was observed in the development of optic vesicles reversely transplanted around the 10-somite stage: the optic cup formation was disturbed and subsequently the neural retina and pigment epithelium did not develop normally. We concluded that establishment of the D-V polarity in the optic vesicle plays an essential role in the patterning and differentiation of the neural retina and pigment epithelium.
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DOI：
10.1073/pnas.95.18.10643
发表时间：
1998
影响因子：
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作者：
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味蕾从内胚层自主发育，无需头神经嵴或轴旁中胚层的诱导。
DOI：
10.1242/dev.124.5.949
发表时间：
1997
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
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通讯作者：
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DOI：
10.1242/dev.126.2.397
发表时间：
1999
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Dale,K;Sattar,N;Heemskerk,J;Clarke,JD;Placzek,M;Dodd,J
通讯作者：
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