Fucoidan suppresses endocytosis in cultured HeLa cells

Fucoidan suppresses endocytosis in cultured HeLa cells
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岩藻依聚糖抑制培养的 HeLa 细胞的内吞作用

DOI:
10.1007/s11655-011-0797-9
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发表时间:
2011-08
影响因子:
2.9
通讯作者:
吴鸿
吴鸿
中科院分区:
医学3区
文献类型:
--
作者:
吴鸿

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目的探讨岩藻糖胶对体外培养的HeLa细胞内吞作用的影响:采用活细胞成像技术。方法:用荧光探针FM1-43和无机荧光量子点(Q-Dot)观察岩藻糖胶对体外培养的HeLa细胞内吞作用的影响。方法:利用荧光探针FM1-43和无机荧光量子点(Q-Dot)观察岩藻糖胶对HeLa细胞内吞作用的影响。结论:褐藻糖胶可抑制HeLa细胞钙依赖的内吞作用,其机制可能与抑制激动剂诱导的钙离子反应有关。
ObjectiveTo evaluate the effects of fucoidan on endocytosis in cultured HeLa cells: in vitro using live cell imaging.MethodsA confocal scanning system and an incubation imaging system were used to: observe the effects of fucoidan on the initial (6 h) stages of endocytosis using the fl uorescent probe FM1-43 and inorganic fl uorescent quantum dot (Q-dots).ResultsAccording to the time-lapse images, fucoidan inhibited the: formation of endocytic vesicles in HeLa cells, in which the FM1-43 dye was entrapped. Fucoidan also had an inhibitory effect on the uptake of the Q-dots by the cell membranes of HeLa cells.ConclusionIt was concluded: that fucoidan suppresses Ca2+-dependent endocytosis in HeLa cells, which may be caused by its inhibitory -effects on agonist-induced Ca2+responses.
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发表时间: 1998-11
期刊: The Journal of Neuroscience
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