GmFLD, a soybean homolog of the autonomous pathway gene FLOWERING LOCUS D, promotes flowering in Arabidopsis thaliana.
GmFLD, a soybean homolog of the autonomous pathway gene FLOWERING LOCUS D, promotes flowering in Arabidopsis thaliana.
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GmFLD 是自主途径基因 FLOWERING LOCUS D 的大豆同源物，可促进拟南芥开花
DOI：
10.1186/s12870-014-0263-x
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发表时间：
2014-10-07
影响因子：
5.3
通讯作者：
Yang W
中科院分区：
文献类型：
--
作者：
Hu Q;Jin Y;Shi H;Yang W
背景：在适当的时间开花对于所有开花植物的种子成熟和繁殖成功至关重要。大豆是典型的短日照植物，大豆基因组中同时存在光周期基因和自主途径基因。然而，大豆自主途径基因的功能却知之甚少。本论文研究了大豆自主途径基因FLOW LOCUS D（FLD）的同源基因GmFLD在拟南芥开花转换中的功能。结果：在大豆中，GmFLD在幼苗的展开子叶、根和幼荚中高度表达。然而，在叶和茎尖中的表达水平较低。GmFLD在A. thaliana（Col）诱导转基因植株提早开花，挽救了拟南芥的晚花表型。拟南芥fld突变体在GmFLD转基因植株（Col或fld背景）中，与相应的非转基因基因型相比，FLC（FLOWERLOCUS C）转录水平降低，而花整合子FT和SOC 1上调。染色质免疫沉淀分析显示，在FLC转录起始位点附近，组蛋白H3 Lys-4的三甲基化和H4的乙酰化水平均显著降低。这与GmFLD作为组蛋白去甲基化酶的功能是一致的。结论：我们的研究结果表明，GmFLD是拟南芥FLD的功能性直系同源物，可能在大豆染色质状态的调控中发挥重要作用。本研究首次为大豆中自主途径组分的进化保守性提供了证据。
Background:Flowering at an appropriate time is crucial for seed maturity and reproductive success in all flowering plants. Soybean (Glycine max) is a typical short day plant, and both photoperiod and autonomous pathway genes exist in soybean genome. However, little is known about the functions of soybean autonomous pathway genes. In this article, we examined the functions of a soybean homolog of the autonomous pathway gene FLOWERING LOCUS D (FLD), GmFLD in the flowering transition of A. thaliana.Results:In soybean, GmFLD is highly expressed in expanded cotyledons of seedlings, roots, and young pods. However, the expression levels are low in leaves and shoot apexes. Expression of GmFLD in A. thaliana (Col) resulted in early flowering of the transgenic plants, and rescued the late flowering phenotype of the A. thaliana fld mutant. In GmFLD transgenic plants (Col or fld background), the FLC (FLOWERING LOCUS C) transcript levels decreased whereas the floral integrators, FT and SOC1, were up-regulated when compared with the corresponding non-transgenic genotypes. Furthermore, chromatin immuno-precipitation analysis showed that in the transgenic rescued lines (fld background), the levels of both tri-methylation of histone H3 Lys-4 and acetylation of H4 decreased significantly around the transcriptional start site of FLC. This is consistent with the function of GmFLD as a histone demethylase.Conclusions:Our results suggest that GmFLD is a functional ortholog of the Arabidopsis FLD and may play an important role in the regulation of chromatin state in soybean. The present data provides the first evidence for the evolutionary conservation of the components in the autonomous pathway in soybean.
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影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
6.9
作者：
Jiang S;Kumar S;Eu YJ;Jami SK;Stasolla C;Hill RD
通讯作者：
Hill RD
DOI：
10.1111/j.1365-313x.2007.03359.x
发表时间：
2008-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jin JB;Jin YH;Lee J;Miura K;Yoo CY;Kim WY;Van Oosten M;Hyun Y;Somers DE;Lee I;Yun DJ;Bressan RA;Hasegawa PM
通讯作者：
Hasegawa PM
影响因子：
5.6
作者：
Bashton, M;Chothia, C
通讯作者：
Chothia, C
影响因子：
4.9
作者：
Jang, Yun Hee;Park, Hyo-Young;Kim, Jeong-Kook
通讯作者：
Kim, Jeong-Kook