Tobramycin at subinhibitory concentration inhibits the RhlI/R quorum sensing system in a Pseudomonas aeruginosa environmental isolate.
Tobramycin at subinhibitory concentration inhibits the RhlI/R quorum sensing system in a Pseudomonas aeruginosa environmental isolate.
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DOI：
10.1186/1471-2334-10-148
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发表时间：
2010-06-02
影响因子：
3.7
通讯作者：
Maravić-Vlahovicek G
中科院分区：
文献类型：
--
作者：
Babić F;Venturi V;Maravić-Vlahovicek G
抗生素不仅是具有杀死或抑制微生物生长的治疗活性的小分子，而且还可以作为影响细菌群落中基因表达的信号分子。一些研究已经证明妥布霉素作为信号分子在转录水平上对基因表达的影响及其对细菌生理和毒力的影响。这些研究表明，妥布霉素的亚抑制浓度（SIC）可诱导生物膜形成，并增强铜绿假单胞菌在特定环境中定殖的能力。环境铜绿假单胞菌菌株PUPa 3在不同浓度的妥布霉素存在下生长，并确定在哪个最高浓度SIC、生长、总蛋白水平和翻译效率不受影响。在SIC，然后确定与细胞-细胞信号传导（称为群体感应）相关的表型是否改变。在本研究中，确定了在SICs处的妥布霉素感测/响应是否影响环境铜绿假单胞菌菌株中的两个独立阿勒QS系统。有理由认为铜绿假单胞菌在自然界中遇到妥布霉素，因为妥布霉素是由生态位伴侣黑暗链霉菌产生的。已确定妥布霉素的SICs通过降低C4-HSL的产生水平来抑制RhlI/R系统。这种效应不是由于rhlI转录的减少，而是需要妥布霉素-核糖体相互作用。在铜绿假单胞菌中发生妥布霉素信号传导，并且不同的菌株可以具有不同的响应。了解环境铜绿假单胞菌对妥布霉素的反应将突出自然界中可能发生的种间信号传导，并可能对人类使用这种非常重要的抗生素的利用模式产生重要影响。
Antibiotics are not only small molecules with therapeutic activity in killing or inhibiting microbial growth, but can also act as signaling molecules affecting gene expression in bacterial communities. A few studies have demonstrated the effect of tobramycin as a signal molecule on gene expression at the transcriptional level and its effect on bacterial physiology and virulence. These have shown that subinhibitory concentrations (SICs) of tobramycin induce biofilm formation and enhance the capabilities of P. aeruginosa to colonize specific environments. Environmental P. aeruginosa strain PUPa3 was grown in the presence of different concentrations of tobramycin and it was determined at which highest concentration SIC, growth, total protein levels and translation efficiency were not affected. At SIC it was then established if phenotypes related to cell-cell signaling known as quorum sensing were altered. In this study it was determined whether tobramycin sensing/response at SICs was affecting the two independent AHL QS systems in an environmental P. aeruginosa strain. It is reasonable to assume that P. aeruginosa encounters tobramycin in nature since it is produced by niche mate Streptomyces tenebrarius. It was established that SICs of tobramycin inhibited the RhlI/R system by reducing levels of C4-HSL production. This effect was not due to a decrease of rhlI transcription and required tobramycin-ribosome interaction. Tobramycin signaling in P. aeruginosa occurs and different strains can have a different response. Understanding the tobramycin response by an environmental P. aeruginosa will highlight possible inter-species signalling taking place in nature and can possible also have important implications in the mode of utilization for human use of this very important antibiotic.
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影响因子：
3.2
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通讯作者：
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