Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair.
Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair.
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DOI：
10.3389/fcell.2021.788596
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发表时间：
2021
影响因子：
5.5
通讯作者：
Hardman MJ
中科院分区：
文献类型：
--
作者：
Wilkinson HN;Guinn BA;Hardman MJ
所有生命都需要内源性金属，协调对组织功能至关重要的各种细胞过程的行动。动态伤口愈合反应是由大量的这种细胞行为支撑的，以时间依赖性的方式发生。然而，内源性金属对皮肤修复的重要性在很大程度上仍未被探索。在这里，我们结合联合收割机电感耦合等离子体质谱与组织水平的RNA测序，揭示了深刻的变化，在一些金属，和相应的金属调节基因，在小鼠的时间愈合。创伤钙、镁、铁、铜和锰在创伤后7天升高，而镁、铁、铝、锰和钴在创伤后14天增加。在转录水平上，创伤诱导的途径独立地高度富集金属调控的基因，反之亦然。此外，特定的金属与不同的伤口诱导的生物过程有关，并聚集在小鼠和人类的关键转录调控因子上。最后，我们揭示了一个新发现的转录调节因子，TNF，在钙诱导的表皮分化的潜在作用。总之，这些数据突出了金属在皮肤伤口修复中潜在的新的和多样化的作用，为进一步研究阐明金属在皮肤和其他组织修复中对细胞过程的贡献铺平了道路。
Endogenous metals are required for all life, orchestrating the action of diverse cellular processes that are crucial for tissue function. The dynamic wound healing response is underpinned by a plethora of such cellular behaviours, occurring in a time-dependent manner. However, the importance of endogenous metals for cutaneous repair remains largely unexplored. Here we combine ICP-MS with tissue-level RNA-sequencing to reveal profound changes in a number of metals, and corresponding metal-regulated genes, across temporal healing in mice. Wound calcium, magnesium, iron, copper and manganese are elevated at 7 days post-wounding, while magnesium, iron, aluminium, manganese and cobalt increase at 14 days post-wounding. At the level of transcription, wound-induced pathways are independently highly enriched for metal-regulated genes, and vice versa. Moreover, specific metals are linked to distinct wound-induced biological processes and converge on key transcriptional regulators in mice and humans. Finally, we reveal a potential role for one newly identified transcriptional regulator, TNF, in calcium-induced epidermal differentiation. Together, these data highlight potential new and diverse roles for metals in cutaneous wound repair, paving the way for further studies to elucidate the contribution of metals to cellular processes in the repair of skin and other tissues.
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影响因子：
64.5
作者：
Haldar M;Kohyama M;So AY;Kc W;Wu X;Briseño CG;Satpathy AT;Kretzer NM;Arase H;Rajasekaran NS;Wang L;Egawa T;Igarashi K;Baltimore D;Murphy TL;Murphy KM
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Murphy KM
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11.4
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3
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Thornton, Janet M.
影响因子：
20.3
作者：
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通讯作者：
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影响因子：
2.7
作者：
Bianco, C.;Adami, G.;Maina, G.
通讯作者：
Maina, G.