An efficient microinjection method to generate human anaplasmosis agent Anaplasma phagocytophilum-infected ticks.
An efficient microinjection method to generate human anaplasmosis agent Anaplasma phagocytophilum-infected ticks.
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DOI：
10.1038/s41598-020-73061-9
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发表时间：
2020-09-29
影响因子：
4.6
通讯作者：
Neelakanta G
中科院分区：
文献类型：
--
作者：
Taank V;Ramasamy E;Sultana H;Neelakanta G
蜱是传播包括人无形体病病原体、嗜吞噬细胞无形体在内的多种病原体的重要媒介。这种细菌是一种专性胞内立克次体病原体。通常需要受感染的储库动物宿主来维持该细菌菌落，并作为血液来源在蜱饲养研究中对幼稚动物进行针接种。在这项研究中，我们报告了一种有效的显微注射方法，以产生A。实验室条件下嗜吞噬细胞菌感染的蜱。密核（DC）型A.从体外培养物中分离出嗜吞噬细胞蜱，并将其注射到未进食的未感染肩突硬蜱若虫蜱的肛门中。这些滴答声成功地传输了A。嗜吞噬细胞菌对鼠宿主的作用。在小鼠血液、脾和肝组织中检测细菌载量。此外，蜱幼虫成功地获得了A.来自先前通过喂食DC-显微注射的若虫蜱而感染的小鼠的嗜吞噬细胞菌。A.从幼虫到若虫期的嗜吞噬细胞菌在这些蜱中也是明显的。总之，我们的研究提供了一个及时，快速，有效的方法，不仅产生A。此外，它还提供了一种工具，以了解这种细菌和可能的其他立克次体病原体从医学上重要的载体的获取和传播动力学。
Ticks are important vectors that transmit several pathogens including human anaplasmosis agent, Anaplasma phagocytophilum. This bacterium is an obligate intracellular rickettsial pathogen. An infected reservoir animal host is often required for maintenance of this bacterial colony and as a source for blood to perform needle inoculations in naïve animals for tick feeding studies. In this study, we report an efficient microinjection method to generate A. phagocytophilum-infected ticks in laboratory conditions. The dense-core (DC) form of A. phagocytophilum was isolated from in vitro cultures and injected into the anal pore of unfed uninfected Ixodes scapularis nymphal ticks. These ticks successfully transmitted A. phagocytophilum to the murine host. The bacterial loads were detected in murine blood, spleen, and liver tissues. In addition, larval ticks successfully acquired A. phagocytophilum from mice that were previously infected by feeding with DC-microinjected nymphal ticks. Transstadial transmission of A. phagocytophilum from larvae to nymphal stage was also evident in these ticks. Taken together, our study provides a timely, rapid, and an efficient method not only to generate A. phagocytophilum-infected ticks but also provides a tool to understand acquisition and transmission dynamics of this bacterium and perhaps other rickettsial pathogens from medically important vectors.
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影响因子：
4.5
作者：
Ramasamy, Ellango;Taank, Vikas;Neelakanta, Girish
通讯作者：
Neelakanta, Girish
影响因子：
6.4
作者：
Bunnell, JE;Trigiani, ER;Dumler, JS
通讯作者：
Dumler, JS
影响因子：
3.8
作者：
Grabarek, Adam D.;Weinbuch, Daniel;Hawe, Andrea
通讯作者：
Hawe, Andrea
影响因子：
2.1
作者：
Baldridge GD;Scoles GA;Burkhardt NY;Schloeder B;Kurtti TJ;Munderloh UG
通讯作者：
Munderloh UG
影响因子：
9.4
作者：
Munderloh, UG;Jauron, SD;Goodman, JL
通讯作者：
Goodman, JL